Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tanizaki, M.M.; Zingali, R.B.; Kawazaki, H.; Imajoh, S.; Yamazaki, S.; Suzuki, K.
    Purification and some characteristics of a zinc metalloprotease from the venom of Bothrops jararaca (jararaca) (1989), Toxicon, 27, 747-755.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.50 1,10-phenanthroline + 4 mM Ca2+: 85% inhibition Bothrops jararaca
3.4.24.50 5,5'-dithiobis(2-nitrobenzoic acid) 1 mM: 70% inhibition, in presence of 2 mM Ca2+: 30% inhibition Bothrops jararaca
3.4.24.50 EDTA 0.1 mM: complete inhibition Bothrops jararaca
3.4.24.50 additional information phosphoramidon, leupeptin, epoxysuccinyl derivatives e.g. E-64, Ep-475 or Ep-479, all 0.5 mM, are no inhibitors Bothrops jararaca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.50 Ca2+ activates Bothrops jararaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.50 23000
-
 1 * 23000, Bothrops jararaca, SDS-PAGE Bothrops jararaca

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.50 Bothrops jararaca
-
 jararaca snake
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.50 partial Bothrops jararaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.50 venom
-
 Bothrops jararaca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.50 angiotensin I + H2O cleavage of Pro-Phe Bothrops jararaca Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
-
 ?
3.4.24.50 Insulin B-chain + H2O cleavage of Gln4-His, Ser9-His and Ala14-Leu Bothrops jararaca Hydrolyzed insulin B-chain
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.50 monomer 1 * 23000, Bothrops jararaca, SDS-PAGE Bothrops jararaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.50 37
-
 assay at Bothrops jararaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.50 8.8
-
 assay at Bothrops jararaca