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Literature summary extracted from

  • Duckworth, W.C.; Heinemann, M.A.; Kitabchi, A.E.
    Purification of insulin-specific protease by affinity chromatography (1972), Proc. Natl. Acad. Sci. USA, 69, 3698-3702.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.56 DTT stimulates Rattus norvegicus
3.4.24.56 glutathione stimulates Rattus norvegicus
3.4.24.56 Sulfhydryl-dependent enzyme
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.56 NEM
-
 Rattus norvegicus
3.4.24.56 p-hydroxymercuribenzoate
-
 Rattus norvegicus
3.4.24.56 Proinsulin
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.56 0.000022
-
 Insulin
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.56
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.56 skeletal muscle
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.56 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.24.56 4°C or frozen, unstable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 Insulin + H2O
-
 Rattus norvegicus Hydrolyzed insulin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.56 7.4
-
-
 Rattus norvegicus