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Literature summary extracted from

  • Garcia, J.V.; Fenton, B.W.; Rosner, M.R.
    Isolation and characterization of an insulin-degrading enzyme from Drosophila melanogaster (1988), Biochemistry, 27, 4237-4244.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.56 bacitracin
-
 Drosophila melanogaster
3.4.24.56 additional information not: aprotinin; not: pancreatic trypsin inhibitor Drosophila melanogaster
3.4.24.56 NEM
-
 Drosophila melanogaster
3.4.24.56 PCMB
-
 Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.56 0.000003
-
 Insulin procine substrate Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.56 cytosol
-
 Drosophila melanogaster 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.56 110000
-
 Drosophila melanogaster, gel filtration Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.56 insulin + H2O Drosophila melanogaster important role in the metabolism of insulin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Drosophila melanogaster
-
 Kc cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.56
-
 Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.56 Kc cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 Insulin + H2O not: individual A and B-chains of insulin Drosophila melanogaster Hydrolyzed insulin
-
 ?
3.4.24.56 Insulin + H2O equine Drosophila melanogaster Hydrolyzed insulin
-
 ?
3.4.24.56 insulin + H2O important role in the metabolism of insulin Drosophila melanogaster ?
-
 ?
3.4.24.56 Insulin growth factor II + H2O
-
 Drosophila melanogaster ?
-
 ?
3.4.24.56 additional information insulin-like growth factor I Drosophila melanogaster ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.56 monomer 1 * 110000, Drosophila melanogaster, SDS-PAGE Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.56 37
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.56 7 8
-
 Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.56 6.5 8.5 pH 6.5: about 30% of activity maximum, pH 8.5: about 60% of activity maximum Drosophila melanogaster