EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.60 | (24-28)ANF pentapeptide | i.e. Asn-Ser-Phe-Arg-Tyr, weak, substance P as substrate, inhibits cleavage of Gly9-Leu10 and Phe7-Phe8 bonds | Xenopus laevis | |
3.4.24.60 | (25-28)ANF tetrapeptide | i.e. Ser-Phe-Arg-Tyr, weak, substance P as substrate, inhibits cleavage of Gly9-Leu10 and Phe7-Phe8 bonds | Xenopus laevis | |
3.4.24.60 | 1,10-phenanthroline | 0.01 or 0.05 mM Zn2+, Ca2+ or Mn2+ fully restores; metalloendopeptidase character | Xenopus laevis | |
3.4.24.60 | beta(25-35)Amyloid peptide | i.e. Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met | Xenopus laevis | |
3.4.24.60 | beta(31-35)Amyloid pentapeptide | i.e. Ile-Ile-Gly-Leu-Met | Xenopus laevis | |
3.4.24.60 | beta(31-35)Amyloid-NH2 | i.e. Ile-Ile-Gly-Leu-Met-NH2 | Xenopus laevis | |
3.4.24.60 | CP-96345 | non-peptide agonist of substance P, substance P as substrate, inhibits cleavage of Gly9-Leu10 bond | Xenopus laevis | |
3.4.24.60 | additional information | TIMPS (tissue inhibitor) | Xenopus laevis | |
3.4.24.60 | RP 67580 | non-peptide agonist of substance P, substance P as substrate, inhibits cleavage of Gly9-Leu10 bond | Xenopus laevis | |
3.4.24.60 | [Ala26]-(21-28)ANF | weak | Xenopus laevis | |
3.4.24.60 | [Leu5]enkephalin | - |
Xenopus laevis | |
3.4.24.60 | [Leu5]enkephalinamide | - |
Xenopus laevis | |
3.4.24.60 | [Val26]-(21-28)ANF | weak | Xenopus laevis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.60 | 0.011 | - |
[Arg0,Leu5,Arg6]enkephalin | - |
Xenopus laevis | |
3.4.24.60 | 0.02 | - |
Substance P | [Leu5,Arg6]enkephalin | Xenopus laevis | |
3.4.24.60 | 0.028 | - |
antibacterial peptide PGLa | - |
Xenopus laevis | |
3.4.24.60 | 0.05 | - |
(5-28)ANF | - |
Xenopus laevis | |
3.4.24.60 | 0.079 | - |
[Leu5,Arg6]enkephalin | - |
Xenopus laevis | |
3.4.24.60 | 0.091 | - |
(19-28)ANF peptide | - |
Xenopus laevis | |
3.4.24.60 | 0.12 | - |
[D-Arg8]-kermit | - |
Xenopus laevis | |
3.4.24.60 | 0.124 | - |
(5-11)Substance P heptapeptide | - |
Xenopus laevis | |
3.4.24.60 | 0.125 | - |
beta-(1-40)amyloid | - |
Xenopus laevis | |
3.4.24.60 | 0.133 | - |
[Arg0,Leu5]enkephalin | - |
Xenopus laevis | |
3.4.24.60 | 0.143 | - |
[Ile26](21-28)ANF octapeptide | (6-11)substance P hexapeptide | Xenopus laevis | |
3.4.24.60 | 0.167 | - |
[Leu5,Ile6]enkephalin | - |
Xenopus laevis | |
3.4.24.60 | 0.2 | - |
[Trp26](21-28)ANF octapeptide | - |
Xenopus laevis | |
3.4.24.60 | 0.208 | - |
(22-28)ANF heptapeptide | - |
Xenopus laevis | |
3.4.24.60 | 0.37 | - |
(23-28)ANF hexapeptide | - |
Xenopus laevis | |
3.4.24.60 | 0.408 | - |
[Leu26](21-28)ANF octapeptide | - |
Xenopus laevis | |
3.4.24.60 | 0.42 | - |
neurokinin | - |
Xenopus laevis | |
3.4.24.60 | 0.513 | - |
(20-28)ANF peptide | - |
Xenopus laevis | |
3.4.24.60 | 0.571 | - |
(21-28)ANF octapeptide | - |
Xenopus laevis | |
3.4.24.60 | 0.6 | - |
[Tyr26](21-28)ANF octapeptide | - |
Xenopus laevis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.60 | additional information | metalloendopeptidase | Xenopus laevis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.60 | additional information | Xenopus laevis | might be involved in inactivation of a great number of peptide hormones | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.60 | Xenopus laevis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.60 | improved procedure | Xenopus laevis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Storage Stability | Organism |
---|---|---|
3.4.24.60 | 4°C, in 10 mM potassium phosphate buffer, pH 7.5, 0.05% Nonidet P-40, at least 3 months | Xenopus laevis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.60 | (19-28)ANF peptide + H2O | i.e. atrial natriuretic factor derived peptide, cleavage site: Gly20-Leu21 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | (20-28)ANF peptide + H2O | i.e. atrial natriuretic factor derived peptide | Xenopus laevis | ? | - |
? | |
3.4.24.60 | (21-28)ANF octapeptide + H2O | i.e. atrial natriuretic factor derived peptide | Xenopus laevis | ? | - |
? | |
3.4.24.60 | (22-28)ANF heptapeptide + H2O | i.e. atrial natriuretic factor derived peptide | Xenopus laevis | ? | - |
? | |
3.4.24.60 | (23-28)ANF hexapeptide + H2O | i.e. atrial natriuretic factor derived peptide | Xenopus laevis | ? | - |
? | |
3.4.24.60 | (5-11)Substance P heptapeptide + H2O | i.e. Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, cleavage site: Gly9-Leu10 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | (5-28)ANF + H2O | - |
Xenopus laevis | ANF(5-25) + Phe26-Arg27-Tyr28 | - |
? | |
3.4.24.60 | (6-11)Substance P hexapeptide + H2O | i.e. Gln-Phe-Phe-Gly-Leu-Met-NH2, cleavage site: Gly9-Leu10 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | angiotensin II + H2O | i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe, cleavage site: Tyr4-Ile5 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Antibacterial peptide PGLa + H2O | i.e. Gly-Met-Ala-Ser-Lys-Ala-Gly-Ala-Ile-Ala-Gly-Lys-Ile-Ala-Lys-Val-Ala-Leu-Lys-Ala-Leu-NH2, a peptide of Xenopus laevis skin secretion, cleavage site: Lys12-Ile13 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | beta(1-40)Amyloid | cleavage site: Gly33-Leu34 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | beta-(1-40)amyloid + H2O | - |
Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | substrate specificity | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | minimal peptide chain length required: 6 amino acids | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | no carboxy or amino peptidase activity | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | substance P-(7-11)-peptide, [Val26]-(21-28)ANF-peptide or [Ala26]-(21-28)ANF-peptide | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | hydrolyzes bonds on amino-terminus of hydrophobic amino acids, thermolysin-like cleavage | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | Xaa-Tyr or Xaa-Trp | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | cleavage sites: Xaa-Phe, Xaa-Leu, Xaa-Ile | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | might be involved in inactivation of a great number of peptide hormones | Xenopus laevis | ? | - |
? | |
3.4.24.60 | neurokinin + H2O | - |
Xenopus laevis | ? | - |
? | |
3.4.24.60 | Neurokinin A + H2O | i.e. substance K or neuromedin L, His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Gly8-Leu9, not Ser5-Phe6 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Somatostatin-14 + H2O | i.e. Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys, cleavage site: Phe6-Phe7 within disulfide bridged segment yielding one single product | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Substance P + H2O | i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Substance P + H2O | major cleavage site: Gly9-Leu10, minor site: Phe7-Phe8 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | [Arg0,Leu5,Arg6]enkephalin + H2O | heptapeptide Arg-Tyr-Gly-Gly-Phe-Leu-Arg, cleavage site: Phe5-Leu6 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | [Arg0,Leu5]enkephalin + H2O | hexapeptide Arg-Tyr-Gly-Gly-Phe-Leu, cleavage site: Phe4-Leu5 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | [D-Arg8]-kermit + H2O | model substrate, derivative of kermit i.e. Asp-Val-Asp-Glu-Arg-Asp-Val-Arg-Gly-Phe-Ala-Ser-Phe-Leu-NH2, cleavage site: Ser12-Phe13 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | [Ile26](21-28)ANF octapeptide + H2O | - |
Xenopus laevis | ? | - |
? | |
3.4.24.60 | [Leu26](21-28)ANF octapeptide + H2O | - |
Xenopus laevis | ? | - |
? | |
3.4.24.60 | [Leu5,Arg6]enkephalin + H2O | hexapeptide Tyr-Gly-Gly-Phe-Leu-Arg, cleavage site: Phe4-Leu5 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | [Leu5,Ile6]enkephalin + H2O | hexapeptide Tyr-Gly-Gly-Phe-Leu-Ile, cleavage site: Phe4-Leu5 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | [Leu5,Lys6]enkephalin + H2O | hexapeptide Tyr-Gly-Gly-Phe-Leu-Lys, cleavage site: Phe4-Leu5 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | [Trp26](21-28)ANF octapeptide + H2O | - |
Xenopus laevis | ? | - |
? | |
3.4.24.60 | [Tyr26](21-28)ANF octapeptide + H2O | - |
Xenopus laevis | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.60 | 37 | - |
assay at | Xenopus laevis |