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Literature summary extracted from

  • Joudiou, C.; Carvalho, K.M.; Camarao, G.; Boussetta, H.; Cohen, P.
    Characterization of the thermolysin-like cleavage of biologically active peptides by Xenopus laevis peptide hormone inactivating enzyme (1993), Biochemistry, 32, 5959-5966.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.60 (24-28)ANF pentapeptide i.e. Asn-Ser-Phe-Arg-Tyr, weak, substance P as substrate, inhibits cleavage of Gly9-Leu10 and Phe7-Phe8 bonds Xenopus laevis
3.4.24.60 (25-28)ANF tetrapeptide i.e. Ser-Phe-Arg-Tyr, weak, substance P as substrate, inhibits cleavage of Gly9-Leu10 and Phe7-Phe8 bonds Xenopus laevis
3.4.24.60 1,10-phenanthroline 0.01 or 0.05 mM Zn2+, Ca2+ or Mn2+ fully restores; metalloendopeptidase character Xenopus laevis
3.4.24.60 beta(25-35)Amyloid peptide i.e. Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met Xenopus laevis
3.4.24.60 beta(31-35)Amyloid pentapeptide i.e. Ile-Ile-Gly-Leu-Met Xenopus laevis
3.4.24.60 beta(31-35)Amyloid-NH2 i.e. Ile-Ile-Gly-Leu-Met-NH2 Xenopus laevis
3.4.24.60 CP-96345 non-peptide agonist of substance P, substance P as substrate, inhibits cleavage of Gly9-Leu10 bond Xenopus laevis
3.4.24.60 additional information TIMPS (tissue inhibitor) Xenopus laevis
3.4.24.60 RP 67580 non-peptide agonist of substance P, substance P as substrate, inhibits cleavage of Gly9-Leu10 bond Xenopus laevis
3.4.24.60 [Ala26]-(21-28)ANF weak Xenopus laevis
3.4.24.60 [Leu5]enkephalin
-
Xenopus laevis
3.4.24.60 [Leu5]enkephalinamide
-
Xenopus laevis
3.4.24.60 [Val26]-(21-28)ANF weak Xenopus laevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.60 0.011
-
[Arg0,Leu5,Arg6]enkephalin
-
Xenopus laevis
3.4.24.60 0.02
-
Substance P [Leu5,Arg6]enkephalin Xenopus laevis
3.4.24.60 0.028
-
antibacterial peptide PGLa
-
Xenopus laevis
3.4.24.60 0.05
-
(5-28)ANF
-
Xenopus laevis
3.4.24.60 0.079
-
[Leu5,Arg6]enkephalin
-
Xenopus laevis
3.4.24.60 0.091
-
(19-28)ANF peptide
-
Xenopus laevis
3.4.24.60 0.12
-
[D-Arg8]-kermit
-
Xenopus laevis
3.4.24.60 0.124
-
(5-11)Substance P heptapeptide
-
Xenopus laevis
3.4.24.60 0.125
-
beta-(1-40)amyloid
-
Xenopus laevis
3.4.24.60 0.133
-
[Arg0,Leu5]enkephalin
-
Xenopus laevis
3.4.24.60 0.143
-
[Ile26](21-28)ANF octapeptide (6-11)substance P hexapeptide Xenopus laevis
3.4.24.60 0.167
-
[Leu5,Ile6]enkephalin
-
Xenopus laevis
3.4.24.60 0.2
-
[Trp26](21-28)ANF octapeptide
-
Xenopus laevis
3.4.24.60 0.208
-
(22-28)ANF heptapeptide
-
Xenopus laevis
3.4.24.60 0.37
-
(23-28)ANF hexapeptide
-
Xenopus laevis
3.4.24.60 0.408
-
[Leu26](21-28)ANF octapeptide
-
Xenopus laevis
3.4.24.60 0.42
-
neurokinin
-
Xenopus laevis
3.4.24.60 0.513
-
(20-28)ANF peptide
-
Xenopus laevis
3.4.24.60 0.571
-
(21-28)ANF octapeptide
-
Xenopus laevis
3.4.24.60 0.6
-
[Tyr26](21-28)ANF octapeptide
-
Xenopus laevis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.60 additional information metalloendopeptidase Xenopus laevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.60 additional information Xenopus laevis might be involved in inactivation of a great number of peptide hormones ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.60 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.60 improved procedure Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.4.24.60 4°C, in 10 mM potassium phosphate buffer, pH 7.5, 0.05% Nonidet P-40, at least 3 months Xenopus laevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.60 (19-28)ANF peptide + H2O i.e. atrial natriuretic factor derived peptide, cleavage site: Gly20-Leu21 Xenopus laevis ?
-
?
3.4.24.60 (20-28)ANF peptide + H2O i.e. atrial natriuretic factor derived peptide Xenopus laevis ?
-
?
3.4.24.60 (21-28)ANF octapeptide + H2O i.e. atrial natriuretic factor derived peptide Xenopus laevis ?
-
?
3.4.24.60 (22-28)ANF heptapeptide + H2O i.e. atrial natriuretic factor derived peptide Xenopus laevis ?
-
?
3.4.24.60 (23-28)ANF hexapeptide + H2O i.e. atrial natriuretic factor derived peptide Xenopus laevis ?
-
?
3.4.24.60 (5-11)Substance P heptapeptide + H2O i.e. Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, cleavage site: Gly9-Leu10 Xenopus laevis ?
-
?
3.4.24.60 (5-28)ANF + H2O
-
Xenopus laevis ANF(5-25) + Phe26-Arg27-Tyr28
-
?
3.4.24.60 (6-11)Substance P hexapeptide + H2O i.e. Gln-Phe-Phe-Gly-Leu-Met-NH2, cleavage site: Gly9-Leu10 Xenopus laevis ?
-
?
3.4.24.60 angiotensin II + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe, cleavage site: Tyr4-Ile5 Xenopus laevis ?
-
?
3.4.24.60 Antibacterial peptide PGLa + H2O i.e. Gly-Met-Ala-Ser-Lys-Ala-Gly-Ala-Ile-Ala-Gly-Lys-Ile-Ala-Lys-Val-Ala-Leu-Lys-Ala-Leu-NH2, a peptide of Xenopus laevis skin secretion, cleavage site: Lys12-Ile13 Xenopus laevis ?
-
?
3.4.24.60 beta(1-40)Amyloid cleavage site: Gly33-Leu34 Xenopus laevis ?
-
?
3.4.24.60 beta-(1-40)amyloid + H2O
-
Xenopus laevis ?
-
?
3.4.24.60 additional information substrate specificity Xenopus laevis ?
-
?
3.4.24.60 additional information minimal peptide chain length required: 6 amino acids Xenopus laevis ?
-
?
3.4.24.60 additional information no carboxy or amino peptidase activity Xenopus laevis ?
-
?
3.4.24.60 additional information substance P-(7-11)-peptide, [Val26]-(21-28)ANF-peptide or [Ala26]-(21-28)ANF-peptide Xenopus laevis ?
-
?
3.4.24.60 additional information hydrolyzes bonds on amino-terminus of hydrophobic amino acids, thermolysin-like cleavage Xenopus laevis ?
-
?
3.4.24.60 additional information Xaa-Tyr or Xaa-Trp Xenopus laevis ?
-
?
3.4.24.60 additional information cleavage sites: Xaa-Phe, Xaa-Leu, Xaa-Ile Xenopus laevis ?
-
?
3.4.24.60 additional information might be involved in inactivation of a great number of peptide hormones Xenopus laevis ?
-
?
3.4.24.60 neurokinin + H2O
-
Xenopus laevis ?
-
?
3.4.24.60 Neurokinin A + H2O i.e. substance K or neuromedin L, His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Gly8-Leu9, not Ser5-Phe6 Xenopus laevis ?
-
?
3.4.24.60 Somatostatin-14 + H2O i.e. Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys, cleavage site: Phe6-Phe7 within disulfide bridged segment yielding one single product Xenopus laevis ?
-
?
3.4.24.60 Substance P + H2O i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 Xenopus laevis ?
-
?
3.4.24.60 Substance P + H2O major cleavage site: Gly9-Leu10, minor site: Phe7-Phe8 Xenopus laevis ?
-
?
3.4.24.60 [Arg0,Leu5,Arg6]enkephalin + H2O heptapeptide Arg-Tyr-Gly-Gly-Phe-Leu-Arg, cleavage site: Phe5-Leu6 Xenopus laevis ?
-
?
3.4.24.60 [Arg0,Leu5]enkephalin + H2O hexapeptide Arg-Tyr-Gly-Gly-Phe-Leu, cleavage site: Phe4-Leu5 Xenopus laevis ?
-
?
3.4.24.60 [D-Arg8]-kermit + H2O model substrate, derivative of kermit i.e. Asp-Val-Asp-Glu-Arg-Asp-Val-Arg-Gly-Phe-Ala-Ser-Phe-Leu-NH2, cleavage site: Ser12-Phe13 Xenopus laevis ?
-
?
3.4.24.60 [Ile26](21-28)ANF octapeptide + H2O
-
Xenopus laevis ?
-
?
3.4.24.60 [Leu26](21-28)ANF octapeptide + H2O
-
Xenopus laevis ?
-
?
3.4.24.60 [Leu5,Arg6]enkephalin + H2O hexapeptide Tyr-Gly-Gly-Phe-Leu-Arg, cleavage site: Phe4-Leu5 Xenopus laevis ?
-
?
3.4.24.60 [Leu5,Ile6]enkephalin + H2O hexapeptide Tyr-Gly-Gly-Phe-Leu-Ile, cleavage site: Phe4-Leu5 Xenopus laevis ?
-
?
3.4.24.60 [Leu5,Lys6]enkephalin + H2O hexapeptide Tyr-Gly-Gly-Phe-Leu-Lys, cleavage site: Phe4-Leu5 Xenopus laevis ?
-
?
3.4.24.60 [Trp26](21-28)ANF octapeptide + H2O
-
Xenopus laevis ?
-
?
3.4.24.60 [Tyr26](21-28)ANF octapeptide + H2O
-
Xenopus laevis ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.60 37
-
assay at Xenopus laevis