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Literature summary extracted from

  • D'Auria, S.; Morana, A.; Febbraio, F.; Vaccaro, C.; De Rosa, M.; Nucci, R.
    Functional and structural properties of the homogeneous beta-glycosidase from the extreme thermoacidophilic archaeon Sulfolobus solfataricus expressed in Saccharomyces cerevisiae (1996), Protein Expr. Purif., 7, 299-308.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.B26 analysis reporter genes in molecular biology Saccharolobus solfataricus
3.2.1.B26 nutrition catalyst of lactose hydrolysis in dairy products in the food industry Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B26 expressed in Saccharomyces cerevisiae Saccharolobus solfataricus
3.2.1.B26 expression in Saccharomyces cerevisiae Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 0.3
-
 4-nitrophenyl-cellobioside
-
 Saccharolobus solfataricus
3.2.1.B26 0.3
-
 4-nitrophenyl cellobioside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
3.2.1.B26 0.3
-
 4-nitrophenyl cellobioside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.5
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Saccharolobus solfataricus
3.2.1.B26 0.5
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
3.2.1.B26 0.5
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.7
-
 4-nitrophenyl-beta-D-fucoside
-
 Saccharolobus solfataricus
3.2.1.B26 0.7
-
 4-nitrophenyl beta-D-fucoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
3.2.1.B26 1
-
 4-nitrophenyl beta-D-fucoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1.4
-
 laminaribiose
-
 Saccharolobus solfataricus
3.2.1.B26 3.9
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Saccharolobus solfataricus
3.2.1.B26 3.9
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
3.2.1.B26 4
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 4.3
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Saccharolobus solfataricus
3.2.1.B26 4.3
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
3.2.1.B26 4.7
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 31.5
-
 cellobiose
-
 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B26 60000
-
 4 * 60000, SDS-PAGE Saccharolobus solfataricus
3.2.1.B26 240000
-
 gel filtration Saccharolobus solfataricus
3.2.1.B26 240000
-
 gel filtration, glycerol gradient centrifugation Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.B26 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Saccharolobus solfataricus laminaribiose beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B26
-
 Saccharolobus solfataricus
3.2.1.B26 to homogeneity, recombinant enzyme Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B26 166
-
-
 Saccharolobus solfataricus
3.2.1.B26 166
-
 pH 6.5, 75°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B26 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Saccharolobus solfataricus beta-D-glucose + D-glucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-cellobioside + 2 H2O
-
 Saccharolobus solfataricus 4-nitrophenol + 2 beta-D-glucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-fucoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-galactoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.B26 4-nitrophenyl cellobioside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + cellobiose
-
 ?
3.2.1.B26 additional information hydrolase with broad specificity and noticeable glucan 1 -> 4-beta-D-glucosidase activity Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B26 tetramer 4 * 60000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 Sbetagly
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B26 80
-
 steady increase in activity up to 80°C Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B26 75
-
 5 h, 17% loss of activity Saccharolobus solfataricus
3.2.1.B26 85
-
 83% residual activity after 5 h Saccharolobus solfataricus
3.2.1.B26 85
-
 5 h, 43% loss of activity Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B26 448
-
 4-nitrophenyl cellobioside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
3.2.1.B26 503
-
 4-nitrophenyl beta-D-fucoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
3.2.1.B26 503
-
 4-nitrophenyl cellobioside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 542
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Saccharolobus solfataricus
3.2.1.B26 542
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
3.2.1.B26 542
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 772
-
 4-nitrophenyl beta-D-fucoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1020
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1239
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1285
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
3.2.1.B26 1713
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B26 7
-
-
 Saccharolobus solfataricus
3.2.1.B26 7
-
 in 50 mM sodium phosphate buffer, substrate: 2-nitrophenyl beta-D-galactopyranoside Saccharolobus solfataricus
3.2.1.B26 7
-
 in 50 mM sodium phosphate buffer, substrate: 2-nitrophenyl-beta-D-galactopyranoside Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B26 5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.B26 Saccharolobus solfataricus isoelectric focusing
-
 4