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Literature summary extracted from

  • Saha, B.C.; Bothast, R.J.
    Production, purification, and characterization of a highly glucose-tolerant novel beta-glucosidase from Candida peltata (1996), Appl. Environ. Microbiol., 62, 3165-3170.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 ethanol 11% stimulation at 0.75%, v/v Nakazawaea peltata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 beta-D-glucose highly tolerant to glucose inhibition, Ki: 1.4 M Nakazawaea peltata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 2.3
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Nakazawaea peltata
3.2.1.21 18
-
 cellohexaose
-
 Nakazawaea peltata
3.2.1.21 21
-
 cellopentaose
-
 Nakazawaea peltata
3.2.1.21 35
-
 cellotetraose
-
 Nakazawaea peltata
3.2.1.21 39
-
 cellotriose
-
 Nakazawaea peltata
3.2.1.21 66
-
 cellobiose
-
 Nakazawaea peltata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular best enzyme synthesis if cells grown on glucose Nakazawaea peltata
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 43000
-
 gel filtration Nakazawaea peltata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Nakazawaea peltata cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellohexaose + 5 H2O Nakazawaea peltata
-
 6 D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O Nakazawaea peltata
-
 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O Nakazawaea peltata
-
 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O Nakazawaea peltata
-
 3 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Nakazawaea peltata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography steps Nakazawaea peltata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Nakazawaea peltata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 108
-
-
 Nakazawaea peltata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Nakazawaea peltata 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Nakazawaea peltata beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Nakazawaea peltata 2 beta-D-glucose
-
 ?
3.2.1.21 cellohexaose + 5 H2O
-
 Nakazawaea peltata 6 D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O
-
 Nakazawaea peltata 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Nakazawaea peltata 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O
-
 Nakazawaea peltata 3 D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 43000, SDS-PAGE Nakazawaea peltata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Nakazawaea peltata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Nakazawaea peltata