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Literature summary extracted from

  • Takagi, Y.; Horecker, B.L.
    Purification and properties of a bacterial riboside riboside hydrolase (1956), J. Biol. Chem., 225, 77-86.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.2.1 much activity is lost on lyophilization or dialysis even against the alkaline buffers Lactobacillus delbrueckii

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Lactobacillus delbrueckii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1
-
 Lactobacillus delbrueckii

Storage Stability

EC Number Storage Stability Organism
3.2.2.1 in frozen state, stable for more than 6 months Lactobacillus delbrueckii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 2,6-diamino-9-beta-D-ribofuranosylpurine + H2O 76% of the activity with inosine Lactobacillus delbrueckii 2,6-diaminopurine + D-ribose
-
 ir
3.2.2.1 2,8-diamino-9-beta-D-ribofuranosyladenine + H2O 38% of the activity with inosine Lactobacillus delbrueckii 2,8-diaminoadenine + D-ribose
-
 ir
3.2.2.1 2-hydroxy-9-beta-D-ribofuranosyladenine + H2O 10% of the activity with inosine Lactobacillus delbrueckii 2-hydroxyadenine + D-ribose
-
 ir
3.2.2.1 5,6-dimethyl-1-beta-D-ribofuranosylbenzimidazole + H2O 200% of the activity with inosine Lactobacillus delbrueckii 5,6-dimethylbenzimidazole + D-ribose
-
 ir
3.2.2.1 6-dimethylamino-9-beta-D-ribofuranosylbenzimidazole + H2O 30% of the activity with inosine Lactobacillus delbrueckii 6-dimethylaminobenzimidazole + D-ribose
-
 ir
3.2.2.1 9-beta-D-ribofuranosylpurine + H2O 47% of the activity with inosine Lactobacillus delbrueckii purine + D-ribose
-
 ir
3.2.2.1 adenosine + H2O 103% of the activity with inosine Lactobacillus delbrueckii D-ribose + adenine
-
 ir
3.2.2.1 cytidine + H2O 54% of the activity with inosine Lactobacillus delbrueckii D-ribose + cytosine
-
 ir
3.2.2.1 guanosine + H2O 90% of the activity with inosine Lactobacillus delbrueckii D-ribose + guanine
-
 ir
3.2.2.1 imidazoleacetic acid riboside + H2O 5% of the activity with inosine Lactobacillus delbrueckii imidazoleacetate + D-ribose
-
 ir
3.2.2.1 inosine + H2O
-
 Lactobacillus delbrueckii hypoxanthine + D-ribose
-
 ir
3.2.2.1 nicotinamide riboside + H2O 150% of the activity with inosine Lactobacillus delbrueckii nicotinamide + D-ribose
-
 ir
3.2.2.1 uridine + H2O
-
 Lactobacillus delbrueckii D-ribose + uracil
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.1 6.5
-
 hydrolysis of inosine or uridine Lactobacillus delbrueckii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.2.1 6.2
-
 unstable Lactobacillus delbrueckii
3.2.2.1 7.5
-
 stable Lactobacillus delbrueckii
3.2.2.1 8.6
-
 stable Lactobacillus delbrueckii