Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Joshi, J.G.
    Phosphoglucomutase from yeast (1982), Methods Enzymol., 89, 599-605.
    View publication on PubMed

General Stability

EC Number General Stability Organism
5.4.2.2 stable in 5 mM acetate buffer or 10 mM citrate buffer, pH 5.5, 4 C, but in 5 mM Tris-HCl buffer pH 7.4, 80% loss of activity per day Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.2.2 5,5'-dithiobis(2-nitrobenzoate)
-
 Saccharomyces cerevisiae
5.4.2.2 p-chloromercuribenzoate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.2 Mg2+
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.2 65600
-
 gel filtration, sucrose density gradient centrifugation, sedimentation equilibrium centrifugation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.4.2.2 additional information phosphoprotein, 1 mol phosphate per mol of enzyme Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.2 205
-
 substrate glucose 1-phosphate Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
5.4.2.2 4°C, saturated ammonium sulfate, 0.1 M Na2HPO4-HCl, pH 7.0, 0.1 mM EDTA, 1 mM Mg2+, 3 months stable Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Saccharomyces cerevisiae alpha-D-glucose 6-phosphate
-
 ?