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Literature summary extracted from

  • Eulberg, D.; Lakner, S.; Golovleva, L.A.; Schlömann, M.
    Characterization of a protocatechuate catabolic gene cluster from Rhodococcus opacus 1CP: evidence for a merged enzyme with 4-carboxymuconolactone-decarboxylating and 3-oxoadipate enol-lactone-hydrolyzing activity (1998), J. Bacteriol., 180, 1072-1081.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.44
-
 Rhodococcus opacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.24 42000
-
 gel filtration Rhodococcus opacus
3.1.1.24 42230
-
 calculated from the DNA sequence Rhodococcus opacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.2 additional information Rhodococcus opacus enzyme of the protocatechuate branch of the beta-ketoadipate pathway ?
-
 ?
5.5.1.2 additional information Rhodococcus opacus 1CP enzyme of the protocatechuate branch of the beta-ketoadipate pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.24 Rhodococcus opacus
-
 erythropolis, 1CP
-
4.1.1.44 Rhodococcus opacus
-
-
-
4.1.1.44 Rhodococcus opacus 1CP
-
-
-
5.5.1.2 Rhodococcus opacus
-
-
-
5.5.1.2 Rhodococcus opacus 1CP
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.24 can not be separated from 4-carboxymuconolactone decarboxylase, EC 4.1.1.44, which catalyzes the preceding reaction. The gene pcaD, which encodes the 3-oxoadipate enol-lactone hydrolase is fused to a second gene pcaC, which encodes the decarboxylase, a protocatechuate catabolic gene cluster Rhodococcus opacus
4.1.1.44 copurification of 3-oxoadipate enol-lactone-hydrolyzing activity, ec 3.1.1.24 and 4-carboxymuconolactone-decarboxylating activity, ec 4.1.1.44 Rhodococcus opacus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.24 3-oxoadipate enol-lactone + H2O = 3-oxoadipate can not be separated from 4-carboxymuconolactone decarboxylase, EC 4.1.1.44, which catalyzes the preceding reaction. The gene pcaD, which encodes the 3-oxoadipate enol-lactone hydrolase is fused to a second gene pcaC, which encodes the decarboxylase, a protocatechuate catabolic gene cluster Rhodococcus opacus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O 3-oxoadipate enol-lactone Rhodococcus opacus 3-oxoadipate
-
 ?
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O 3-oxoadipate enol-lactone Rhodococcus opacus 1CP 3-oxoadipate
-
 ?
4.1.1.44 4-carboxymuconolactone
-
 Rhodococcus opacus 5-oxo-4,5-dihydrofuran-2-acetate + CO2
-
 ?
4.1.1.44 4-carboxymuconolactone
-
 Rhodococcus opacus 1CP 5-oxo-4,5-dihydrofuran-2-acetate + CO2
-
 ?
5.5.1.2 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate i.e. beta-carboxy-cis,cis-muconate Rhodococcus opacus cis-Butadiene-1,2,4-tricarboxylate i.e. gamma-carboxymuconolactone ?
5.5.1.2 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate i.e. beta-carboxy-cis,cis-muconate Rhodococcus opacus 1CP cis-Butadiene-1,2,4-tricarboxylate i.e. gamma-carboxymuconolactone ?
5.5.1.2 additional information enzyme of the protocatechuate branch of the beta-ketoadipate pathway Rhodococcus opacus ?
-
 ?
5.5.1.2 additional information enzyme of the protocatechuate branch of the beta-ketoadipate pathway Rhodococcus opacus 1CP ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.24 3-oxoadipate enol-lactone hydrolase
-
 Rhodococcus opacus