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Literature summary extracted from

  • Vanni, P.; Vicenzini, M.T.; Nerozzi, F.M.; Sinha, S.P.
    Studies on isocitrate lyase isolated from Lupinus cotyledons (1979), Can. J. Biochem., 57, 1131-1137.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 malonate
-
 Lupinus sp.
4.1.3.1 N-ethylmaleimide
-
 Lupinus sp.
4.1.3.1 oxalate
-
 Lupinus sp.
4.1.3.1 p-chloromercuribenzoate
-
 Lupinus sp.
4.1.3.1 phosphoenolpyruvate
-
 Lupinus sp.
4.1.3.1 succinate
-
 Lupinus sp.
4.1.3.1 Tartrate
-
 Lupinus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.1 0.2 isocitrate Tris, triethanolamine or imidazole buffer Lupinus sp.
4.1.3.1 1
-
 isocitrate phosphate buffer, pH 6.8 or 7.5 Lupinus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+
-
 Lupinus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 145000 170000 gel filtration Lupinus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Lupinus sp. glyoxylate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Lupinus sp.
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.3.1 reducing agent necessary for maintenance of enzyme activity Lupinus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Lupinus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.1 cotyledon
-
 Lupinus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 3.68
-
-
 Lupinus sp.

Storage Stability

EC Number Storage Stability Organism
4.1.3.1 -30°C, 0.3 mM dithiothreitol, 12 days, 50% loss of activity Lupinus sp.
4.1.3.1 -30°C, 12 days, almost all activity lost Lupinus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Lupinus sp. succinate + glyoxylate
-
 r
4.1.3.1 isocitrate glyoxylate cycle Lupinus sp. ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 7
-
 triethanolamine buffer Lupinus sp.
4.1.3.1 7.5
-
 phosphate, Tris or imidazole buffer Lupinus sp.