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Literature summary extracted from
- Plant microbody proteins. Purification and glycoprotein nature of glyoxysomal isocitrate lyase from cucumber cotyledons (1978), Eur. J. Biochem., 92, 35-43.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | SO42- | - |
Cucumis sativus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.1 | 0.1 | - |
threo-Ds-isocitrate | Hepes, pH 7.4 | Cucumis sativus | |
| 4.1.3.1 | 0.26 | - |
threo-Ds-isocitrate | Hepes, pH 7.4, 60 mM Cl- | Cucumis sativus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.1.3.1 | glyoxysome | matrix | Cucumis sativus | 9514 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 64000 | - |
4 * 64000, SDS-PAGE | Cucumis sativus |
| 4.1.3.1 | 255000 | - |
gel filtration | Cucumis sativus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.1 | isocitrate | Cucumis sativus | glyoxylate cycle | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | Cucumis sativus | - |
cucumber | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 4.1.3.1 | side-chain modification | 2% neutral sugar | Cucumis sativus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | cotyledon | - |
Cucumis sativus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.1 | (2R,3S)-isocitrate | - |
Cucumis sativus | succinate + glyoxylate | - |
? | |
| 4.1.3.1 | isocitrate | - |
Cucumis sativus | succinate + glyoxylate | - |
r | |
| 4.1.3.1 | isocitrate | glyoxylate cycle | Cucumis sativus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.3.1 | tetramer | 4 * 64000, SDS-PAGE | Cucumis sativus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 7.2 | 7.6 | - |
Cucumis sativus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 6.9 | 7.9 | 50% of optimal activity at pH 6.9 and 7.9 | Cucumis sativus |
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Release 2023.1 (January 2023)
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