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Literature summary extracted from

  • Chell, R.M.; Sundaram, T.K.
    Isolation and characterization of isocitrate lyase and malate synthase from Bacillus stearothermophilus (1975), Biochem. Soc. Trans., 3, 303-306.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 KCl strong activation by KCl Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 0.008
-
 acetyl-CoA
-
 Geobacillus stearothermophilus
2.3.3.9 0.088
-
 acetyl-CoA
-
 Geobacillus stearothermophilus
4.1.3.1 0.021
-
 threo-Ds-isocitrate
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.9 58000
-
 1 * 58000, SDS-PAGE Geobacillus stearothermophilus
2.3.3.9 62000
-
 gel filtration Geobacillus stearothermophilus
4.1.3.1 48000
-
 4 * 48000, SDS-PAGE Geobacillus stearothermophilus
4.1.3.1 180000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Geobacillus stearothermophilus glyoxylate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Geobacillus stearothermophilus
-
-
-
4.1.3.1 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9
-
 Geobacillus stearothermophilus
4.1.3.1
-
 Geobacillus stearothermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.9 culture condition:acetate-grown cell
-
 Geobacillus stearothermophilus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.9 additional information
-
-
 Geobacillus stearothermophilus
4.1.3.1 2.9
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 glyoxylate + acetyl-CoA + H2O
-
 Geobacillus stearothermophilus (S)-malate + CoA
-
 ?
4.1.3.1 (2R,3S)-isocitrate
-
 Geobacillus stearothermophilus succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Geobacillus stearothermophilus succinate + glyoxylate
-
 r
4.1.3.1 isocitrate glyoxylate cycle Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.9 monomer 1 * 58000, SDS-PAGE Geobacillus stearothermophilus
4.1.3.1 tetramer 4 * 48000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.9 60
-
 less than 10% loss of activity after 2 h at pH 8 Geobacillus stearothermophilus
4.1.3.1 55
-
 50% activity lost after 4 h Geobacillus stearothermophilus
4.1.3.1 60
-
 50% activity lost after 3 min Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.9 26.7
-
 acetyl-CoA
-
 Geobacillus stearothermophilus
4.1.3.1 24
-
 isocitrate
-
 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.9 8.6
-
-
 Geobacillus stearothermophilus
4.1.3.1 8
-
-
 Geobacillus stearothermophilus