Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chell, R.M.; Sundaram, T.K.; Wilkinson, A.E.
    Isolation and characterization of isocitrate lyase from a thermophilic Bacillus sp. (1978), Biochem. J., 173, 165-177.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 Maleate condensation reaction Bacillus sp. (in: Bacteria)
4.1.3.1 malonate condensation reaction Bacillus sp. (in: Bacteria)
4.1.3.1 methylenesuccinate condensation reaction Bacillus sp. (in: Bacteria)
4.1.3.1 p-chloromercuribenzoate 100% inhibition at 0.02 mM, reversible by 2-mercaptoethanol Bacillus sp. (in: Bacteria)
4.1.3.1 phosphoenolpyruvate
-
 Bacillus sp. (in: Bacteria)
4.1.3.1 succinate
-
 Bacillus sp. (in: Bacteria)
4.1.3.1 Urea partial inactivation Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.02
-
 threo-Ds-isocitrate 25 mM imidazole buffer, pH 6.8 Bacillus sp. (in: Bacteria)
4.1.3.1 0.2
-
 threo-Ds-isocitrate 0.4 M KCl, 25 mM imidazole buffer, pH 6.8 Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+ absolute requirement Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 48000
-
 4 * 48000, SDS-PAGE Bacillus sp. (in: Bacteria)
4.1.3.1 180000
-
 sedimentation equilibrium centrifugation, gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Bacillus sp. (in: Bacteria) glyoxylate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Bacillus sp. (in: Bacteria)
-
 thermophilic
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 thermophilic Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 2.9
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 (2R,3S)-isocitrate
-
 Bacillus sp. (in: Bacteria) succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Bacillus sp. (in: Bacteria) succinate + glyoxylate
-
 r
4.1.3.1 isocitrate glyoxylate cycle Bacillus sp. (in: Bacteria) ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 tetramer 4 * 48000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.1 additional information
-
 KCl, MgCl2, CaCl2 stabilize Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.1 24
-
 threo-Ds-isocitrate
-
 Bacillus sp. (in: Bacteria)