Any feedback?
Literature summary extracted from
- Isocitrate lyase from Neurospora crassa. I. Purification, kinetic mechanism, and interaction with inhibitors (1974), Biochim. Biophys. Acta, 364, 327-340.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | thiol compounds | increase of activity | Neurospora crassa |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | Cl- | - |
Neurospora crassa | |
| 4.1.3.1 | Cl- | - |
Vogesella indigofera | |
| 4.1.3.1 | fructose-1,6-bisphosphate | - |
Neurospora crassa | |
| 4.1.3.1 | fumarate | - |
Neurospora crassa | |
| 4.1.3.1 | fumarate | - |
Vogesella indigofera | |
| 4.1.3.1 | glyoxylate | - |
Neurospora crassa | |
| 4.1.3.1 | HPO42- | - |
Neurospora crassa | |
| 4.1.3.1 | HPO42- | - |
Vogesella indigofera | |
| 4.1.3.1 | malate | - |
Neurospora crassa | |
| 4.1.3.1 | malate | - |
Vogesella indigofera | |
| 4.1.3.1 | NO3- | - |
Neurospora crassa | |
| 4.1.3.1 | NO3- | - |
Vogesella indigofera | |
| 4.1.3.1 | phosphoenolpyruvate | - |
Neurospora crassa | |
| 4.1.3.1 | SO42- | - |
Neurospora crassa | |
| 4.1.3.1 | SO42- | - |
Vogesella indigofera | |
| 4.1.3.1 | succinate | - |
Neurospora crassa | |
| 4.1.3.1 | succinate | - |
Vogesella indigofera | |
| 4.1.3.1 | threo-DL-homoisocitrate | - |
Neurospora crassa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.1 | 0.014 | - |
glyoxylate | - |
Neurospora crassa | |
| 4.1.3.1 | 0.044 | - |
succinate | - |
Neurospora crassa | |
| 4.1.3.1 | 0.05 | - |
threo-Ds-isocitrate | - |
Neurospora crassa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | Mg2+ | increase of activity | Neurospora crassa | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.1 | isocitrate | Neurospora crassa | glyoxylate cycle | ? | - |
? | |
| 4.1.3.1 | isocitrate | Vogesella indigofera | glyoxylate cycle | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | Neurospora crassa | - |
- |
- |
| 4.1.3.1 | Vogesella indigofera | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.3.1 | - |
Neurospora crassa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 16 | - |
- |
Neurospora crassa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.3.1 | -80°C, 6 months, stable | Neurospora crassa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.1 | (2R,3S)-isocitrate | - |
Neurospora crassa | succinate + glyoxylate | - |
? | |
| 4.1.3.1 | isocitrate | - |
Neurospora crassa | succinate + glyoxylate | - |
r | |
| 4.1.3.1 | isocitrate | - |
Vogesella indigofera | succinate + glyoxylate | - |
r | |
| 4.1.3.1 | isocitrate | glyoxylate cycle | Neurospora crassa | ? | - |
? | |
| 4.1.3.1 | isocitrate | glyoxylate cycle | Vogesella indigofera | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 6.8 | - |
- |
Neurospora crassa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
