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Literature summary extracted from
- Purification and properties of isocitrate lyase from flax seedlings (1977), Arch. Biochem. Biophys., 183, 13-23.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | cysteine | increase of activity | Linum usitatissimum |  |
| 4.1.3.1 | sulfhydryl compounds | increase of activity | Linum usitatissimum | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | glyoxylate | - |
Linum usitatissimum | |
| 4.1.3.1 | malonate | - |
Linum usitatissimum | |
| 4.1.3.1 | sulfhydryl compounds | increase of activity | Linum usitatissimum | |
| 4.1.3.1 | Tartrate | - |
Linum usitatissimum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | Co2+ | no activity in absence of exogenous cation | Linum usitatissimum | |
| 4.1.3.1 | Co2+ | Co2+ 29% as effective as Mg2+ | Linum usitatissimum | |
| 4.1.3.1 | Mg2+ | increase of activity | Linum usitatissimum | |
| 4.1.3.1 | Mn2+ | no activity in absence of exogenous cation | Linum usitatissimum | |
| 4.1.3.1 | Mn2+ | Mn2+ 24% as effective as Mg2+ | Linum usitatissimum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 264000 | - |
gel filtration | Linum usitatissimum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | Linum usitatissimum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.3.1 | - |
Linum usitatissimum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | seedling | - |
Linum usitatissimum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.1 | isocitrate | - |
Linum usitatissimum | succinate + glyoxylate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.3.1 | tetramer | 4*67000, SDS-PAGE | Linum usitatissimum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 30 | - |
- |
Linum usitatissimum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 15 | 40 | - |
Linum usitatissimum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 7.5 | - |
phosphate, Tris or imidazole buffer | Linum usitatissimum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 5 | 10 | - |
Linum usitatissimum |
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Release 2023.1 (January 2023)
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