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Literature summary extracted from

  • Tanaka, Y.; Yoshida, T.; Watanabe, K.; Izumi, Y.; Mitsunaga, T.
    Characterization, gene cloning and expression of isocitrate lyase involved in the assimilation of one-carbon compounds in Hyphomicrobium methylovorum GM2 (1997), Eur. J. Biochem., 249, 820-825.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 expression in Escherichia coli Hyphomicrobium methylovorum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 Ag+
-
 Hyphomicrobium methylovorum
4.1.3.1 Al3+
-
 Hyphomicrobium methylovorum
4.1.3.1 Cd2+
-
 Hyphomicrobium methylovorum
4.1.3.1 DL-penicillamin
-
 Hyphomicrobium methylovorum
4.1.3.1 glycolate 20% inhibition at 1 mM Hyphomicrobium methylovorum
4.1.3.1 iodoacetamide
-
 Hyphomicrobium methylovorum
4.1.3.1 KCl
-
 Hyphomicrobium methylovorum
4.1.3.1 KCN
-
 Hyphomicrobium methylovorum
4.1.3.1 Li+
-
 Hyphomicrobium methylovorum
4.1.3.1 Mn2+
-
 Hyphomicrobium methylovorum
4.1.3.1 NaCl
-
 Hyphomicrobium methylovorum
4.1.3.1 oxalate 77% inhibition at 1 mM Hyphomicrobium methylovorum
4.1.3.1 Sr2+
-
 Hyphomicrobium methylovorum
4.1.3.1 succinate 38% inhibition at 1 mM Hyphomicrobium methylovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.051
-
 threo-Ds-isocitrate
-
 Hyphomicrobium methylovorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Ba2+ no activation Hyphomicrobium methylovorum
4.1.3.1 Mg2+ increase of activity Hyphomicrobium methylovorum
4.1.3.1 Mn2+ Mn2+ 27% as effective as Mg2+ Hyphomicrobium methylovorum
4.1.3.1 Mn2+ no activity in absence of exogenous cation Hyphomicrobium methylovorum
4.1.3.1 Sr2+ no activation Hyphomicrobium methylovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 62000
-
 4 * 62000, SDS-PAGE Hyphomicrobium methylovorum
4.1.3.1 250000
-
 gel filtration Hyphomicrobium methylovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Hyphomicrobium methylovorum assimilation of one-carbon compounds in the icl+ serine pathway ?
-
 ?
4.1.3.1 isocitrate Hyphomicrobium methylovorum glyoxylate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Hyphomicrobium methylovorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 2.4
-
-
 Hyphomicrobium methylovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 (2R,3S)-isocitrate
-
 Hyphomicrobium methylovorum succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Hyphomicrobium methylovorum succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate assimilation of one-carbon compounds in the icl+ serine pathway Hyphomicrobium methylovorum ?
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Hyphomicrobium methylovorum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 tetramer 4 * 62000, SDS-PAGE Hyphomicrobium methylovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.1 45
-
-
 Hyphomicrobium methylovorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.1 25
-
 no loss of activity after 30 min Hyphomicrobium methylovorum
4.1.3.1 30
-
 16% loss of activity after 30 min Hyphomicrobium methylovorum
4.1.3.1 40
-
 30% loss of activity after 30 min Hyphomicrobium methylovorum
4.1.3.1 50
-
 82% loss of activity after 30 min Hyphomicrobium methylovorum
4.1.3.1 60
-
 100% loss of activity after 30 min Hyphomicrobium methylovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 7.5
-
-
 Hyphomicrobium methylovorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.3.1 7.5 9 at 15°C stable for 30 min Hyphomicrobium methylovorum