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Literature summary extracted from
- Soybean isocitrate lyase: Purification, immunoassay and N-terminal sequence (1991), Plant Physiol. Biochem., 29, 119-129.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.1 | 0.093 | - |
DL-isocitrate | - |
Glycine max |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 63000 | - |
2 * 63000, calculation from amino acid sequence | Glycine max |
| 4.1.3.1 | 64000 | - |
2 * 64000, SDS-PAGE | Glycine max |
| 4.1.3.1 | 130000 | - |
gel filtration | Glycine max |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.1 | isocitrate | Glycine max | glyoxylate cycle | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | Glycine max | - |
soybean | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.3.1 | - |
Glycine max |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | cotyledon | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 9.48 | - |
- |
Glycine max |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.3.1 | -12°C, 20% glycerol, 21 days, 20% loss of activity | Glycine max |
| 4.1.3.1 | -12°C, 20% glycerol, 7 days, 20% loss of activity | Glycine max |
| 4.1.3.1 | -12°C, without glycerol, 4 h, 100% loss of activity | Glycine max |
| 4.1.3.1 | 4°C, 20% glycerol, 7 days, 30% loss of activity | Glycine max |
| 4.1.3.1 | 4°C, without glycerol, 7 days, 50% loss of activity | Glycine max |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.1 | isocitrate | - |
Glycine max | succinate + glyoxylate | - |
? | |
| 4.1.3.1 | isocitrate | glyoxylate cycle | Glycine max | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.3.1 | dimer | 2 * 64000, SDS-PAGE | Glycine max |
| 4.1.3.1 | dimer | 2 * 63000, calculation from amino acid sequence | Glycine max |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 50 | - |
- |
Glycine max |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 50 | - |
heating to temperatures above caused loss of activity | Glycine max |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 8 | - |
- |
Glycine max |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 7 | 9 | 30% of optimal activity at pH 7 and pH 9 | Glycine max |
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