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Literature summary extracted from

  • Fukui, T.; Shiomi, N.; Doi, Y.
    Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae (1998), J. Bacteriol., 180, 667-673.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.119 expression in Escherichia coli BL21 (DE3) Aeromonas caviae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.119 0.029
-
crotonyl-CoA pH 8.0, 30°C Aeromonas caviae
4.2.1.119 0.034
-
hex-2-enoyl-CoA pH 8.0, 30°C Aeromonas caviae
4.2.1.119 0.036
-
pent-2-enoyl-CoA pH 8.0, 30°C Aeromonas caviae
4.2.1.119 0.05
-
oct-2-enoyl-CoA pH 8.0, 30°C Aeromonas caviae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.119 13954
-
2 * 13954, calculated from sequence Aeromonas caviae
4.2.1.119 30000
-
gel filtration Aeromonas caviae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.119 additional information Aeromonas caviae channelling pathway for supplying (R)-3-hydroxyacyl-CoA monomer units from fatty acid beta-oxidation to poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.119 Aeromonas caviae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.119 crotonyl-CoA + H2O
-
Aeromonas caviae (R)-3-hydroxybutanoyl-CoA
-
?
4.2.1.119 hex-2-enoyl-CoA + H2O
-
Aeromonas caviae (R)-3-hydroxyhexanoyl-CoA
-
?
4.2.1.119 additional information channelling pathway for supplying (R)-3-hydroxyacyl-CoA monomer units from fatty acid beta-oxidation to poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis Aeromonas caviae ?
-
?
4.2.1.119 oct-2-enoyl-CoA + H2O
-
Aeromonas caviae (R)-3-hydroxyoctanoyl-CoA
-
?
4.2.1.119 pent-2-enoyl-CoA + H2O
-
Aeromonas caviae (R)-3-hydroxypentanoyl-CoA
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.119 dimer 2 * 13954, calculated from sequence Aeromonas caviae

Synonyms

EC Number Synonyms Comment Organism
4.2.1.119 (R)-specific enoyl coenzyme A hydratase
-
Aeromonas caviae
4.2.1.119 phaJ
-
Aeromonas caviae
4.2.1.119 PhaJAc
-
Aeromonas caviae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.119 30
-
assay at Aeromonas caviae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.119 8
-
assay at Aeromonas caviae