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Literature summary extracted from

  • Puchegger, S.; Redl, B.; Stöpfler, G.
    Purification and properties of a thermostable fumarate hydratase from the archaeobacterium Sulfolobus solfataricus (1990), J. Gen. Microbiol., 136, 1537-1541.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.2 50% v/v ethanol, 50% v/v methanol, 50% v/v 2-propanol, 4 M urea or 0.1% SDS, stable after treatment for 10 h at room temperature Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.2 2-propanol 15-20% of maximum activity when assayed in 40% (v/v) 2-propanol Saccharolobus solfataricus
4.2.1.2 ethanol 15-20% of maximum activity when assayed in 40% (v/v) ethanol Saccharolobus solfataricus
4.2.1.2 K3PO4 10 mM Saccharolobus solfataricus
4.2.1.2 methanol 94% of maximum activity when assayed in 40% (v/v) methanol Saccharolobus solfataricus
4.2.1.2 phosphate
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.125
-
 fumarate
-
 Saccharolobus solfataricus
4.2.1.2 0.125
-
 fumarate pH 7.5, 70°C Saccharolobus solfataricus
4.2.1.2 0.3
-
 (S)-malate
-
 Saccharolobus solfataricus
4.2.1.2 0.3
-
 (S)-malate pH 7.5, 70°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 45000
-
 4 * 45000, SDS-PAGE Saccharolobus solfataricus
4.2.1.2 160000
-
 sucrose density gradient centrifugation Saccharolobus solfataricus
4.2.1.2 170000
-
 gel filtration Saccharolobus solfataricus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.2.1.2 2-propanol 50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus
4.2.1.2 Ethanol 50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus
4.2.1.2 Methanol 50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus
4.2.1.2 SDS 0.1% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus
4.2.1.2 urea 0.1% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Saccharolobus solfataricus
-
-
-
4.2.1.2 Saccharolobus solfataricus
-
 DSM 1616
-
4.2.1.2 Saccharolobus solfataricus DSM 1616
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.2
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.2 205
-
-
 Saccharolobus solfataricus
4.2.1.2 205
-
 pH 7.5, 70°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Saccharolobus solfataricus fumarate + H2O
-
 r
4.2.1.2 (S)-malate
-
 Saccharolobus solfataricus DSM 1616 fumarate + H2O
-
 r
4.2.1.2 fumarate + H2O
-
 Saccharolobus solfataricus L-malate
-
 r
4.2.1.2 fumarate + H2O
-
 Saccharolobus solfataricus DSM 1616 L-malate
-
 r
4.2.1.2 additional information no activity with D-malate, maleate, citramalate and citrate Saccharolobus solfataricus ?
-
 ?
4.2.1.2 additional information no activity with D-malate, maleate, citramalate and citrate Saccharolobus solfataricus DSM 1616 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.2 tetramer 4 * 45000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.2 70
-
 assay at Saccharolobus solfataricus
4.2.1.2 85
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.2 85 95 inactive at 40°C, 85°C: optimum, 95°C: 66% of maximal activity Saccharolobus solfataricus
4.2.1.2 85 95 85°C: maximal activity, 95°C: 66% of maximal activity, the enzyme is almost inactive at 40°C Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 70
-
 1 h, stable Saccharolobus solfataricus
4.2.1.2 85
-
 1 h, stable Saccharolobus solfataricus
4.2.1.2 90
-
 30 min, 72% of maximal activity Saccharolobus solfataricus
4.2.1.2 90
-
 30 min, 72% loss of activity Saccharolobus solfataricus
4.2.1.2 100
-
 30 min, complete loss of activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 7.5
-
 assay at Saccharolobus solfataricus
4.2.1.2 8
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.2 5.5 9 pH 5.5: 43% of maximal activity, pH 9.0: 70% of maximal activity Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.2 Saccharolobus solfataricus isoelectric focusing
-
 7.1