Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Barker, H.A.; Kahn, J.M.; Hedrick, L.
    Pathway of lysine degradation in Fusobacterium nucleatum (1982), J. Bacteriol., 152, 201-207.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.247 2-mercaptoethanol
-
 Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 EDTA activates at low concentrations in the presence of a higher concentration of CoCl2, inhibits at 0.1-1.0 mM by 30% Fusobacterium nucleatum subsp. nucleatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.247 EDTA activates at low concentrations in the presence of a higher concentration of CoCl2, inhibits at 0.1-1.0 mM by 30% Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 additional information no inhibition by DTNB Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 potassium phosphate 4, 15, 30, 65, and 85% inhibition at 0.2, 0.4, 1.0, 10, and 100 mM, respectively Fusobacterium nucleatum subsp. nucleatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.247 Co2+ strong activation Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 Mn2+ moderate activation Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 additional information no activation by Mg2+ Fusobacterium nucleatum subsp. nucleatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA Fusobacterium nucleatum subsp. nucleatum
-
 L-3-aminobutanoyl-CoA + acetoacetate
-
 r
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847
-
 L-3-aminobutanoyl-CoA + acetoacetate
-
 r
4.3.1.14 additional information Fusobacterium nucleatum enzyme is involved in the pathway of Lys degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.247 Fusobacterium nucleatum subsp. nucleatum Q8RHX2
-
-
2.3.1.247 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 Q8RHX2
-
-
4.3.1.14 Fusobacterium nucleatum
-
 ATCC 25586
-
5.4.3.2 Fusobacterium nucleatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.247 native enzyme 143fold by anion exchange chromatography Fusobacterium nucleatum subsp. nucleatum
4.3.1.14
-
 Fusobacterium nucleatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.247 0.18
-
 curde enzyme extract, pH 7.0, 25°C Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 15.7
-
 purified enzyme, pH 7.0, 25°C Fusobacterium nucleatum subsp. nucleatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Fusobacterium nucleatum subsp. nucleatum L-3-aminobutanoyl-CoA + acetoacetate
-
 r
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Fusobacterium nucleatum subsp. nucleatum L-3-aminobutanoyl-CoA + acetoacetate DL-3-aminobutanoyl-CoA and acetoacetate are used in the reverse reaction assay r
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 L-3-aminobutanoyl-CoA + acetoacetate
-
 r
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 L-3-aminobutanoyl-CoA + acetoacetate DL-3-aminobutanoyl-CoA and acetoacetate are used in the reverse reaction assay r
2.3.1.247 beta-alanyl-CoA + acetoacetate
-
 Fusobacterium nucleatum subsp. nucleatum ?
-
 r
2.3.1.247 beta-alanyl-CoA + acetoacetate
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 ?
-
 r
2.3.1.247 DL-3-aminobutanoyl-CoA + 2-methylacetoacetate
-
 Fusobacterium nucleatum subsp. nucleatum (5S)-5-amino-2-methyl-3-oxohexanoate + acetyl-CoA
-
 r
2.3.1.247 DL-3-aminobutanoyl-CoA + 2-methylacetoacetate
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 (5S)-5-amino-2-methyl-3-oxohexanoate + acetyl-CoA
-
 r
4.3.1.14 L-3-Aminobutanoyl-CoA
-
 Fusobacterium nucleatum Crotonyl-CoA + NH3
-
 ?
4.3.1.14 additional information enzyme is involved in the pathway of Lys degradation Fusobacterium nucleatum ?
-
 ?
5.4.3.2 L-Lys
-
 Fusobacterium nucleatum (3S)-3,6-diaminohexanoic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.247 3-keto,5-aminohexanoate cleavage enzyme
-
 Fusobacterium nucleatum subsp. nucleatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.247 6.5 7 forward reaction Fusobacterium nucleatum subsp. nucleatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.247 5.2 8.1 50% of maximal activity at pH 5.2 and pH 8.1 Fusobacterium nucleatum subsp. nucleatum

General Information

EC Number General Information Comment Organism
2.3.1.247 metabolism the enzyme is involved in the the 3-keto-5-aminohexanoate pathway of lysine degradation, tracer experiments on acetate and butyrate formation from lysine and acetate, overview Fusobacterium nucleatum subsp. nucleatum