EC Number | General Stability | Organism |
---|---|---|
4.2.1.6 | insensitive to repeated freeze-thaw cycles | Pseudomonas sp. |
4.2.1.6 | stable to lyophilization in 0.05 M glycine/0.01 M sodium cacodylate, pH 8.8 | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.6 | EDTA | - |
Pseudomonas sp. | |
4.2.1.6 | iodoacetamide | - |
Pseudomonas sp. | |
4.2.1.6 | PCMB | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.6 | 1.14 | - |
D-galactonate | - |
Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.6 | Co2+ | divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+ | Pseudomonas sp. | |
4.2.1.6 | Mg2+ | optimal concentration: 1 mM | Pseudomonas sp. | |
4.2.1.6 | Mg2+ | divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+ | Pseudomonas sp. | |
4.2.1.6 | Mn2+ | divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+ | Pseudomonas sp. | |
4.2.1.6 | Ni2+ | divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+ | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.6 | 57000 | - |
4 * 57000, SDS-PAGE | Pseudomonas sp. |
4.2.1.6 | 240000 | 245000 | gel filtration, sucrose density gradient centrifugation | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.6 | D-galactonate | Pseudomonas sp. | growth on D-galactonate, L-arabinose and D-fucose increases the enzyme activity over the level of cells grown in nutrient broth | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.6 | Pseudomonas sp. | - |
ATCC 27855 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.6 | - |
Pseudomonas sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.1.6 | D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O | the immediate dehydration product is the enol which undergoes spontaneous ketonization | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.6 | 38 | - |
- |
Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.6 | -20°C, 6 months, stable | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.6 | D-galactonate | growth on D-galactonate, L-arabinose and D-fucose increases the enzyme activity over the level of cells grown in nutrient broth | Pseudomonas sp. | ? | - |
? | |
4.2.1.6 | D-galactonate | - |
Pseudomonas sp. | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.6 | tetramer | 4 * 57000, SDS-PAGE | Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.6 | additional information | - |
Mg2+, dithiothreitol and D-galactonate increase the rate of thermal inactivation | Pseudomonas sp. |
4.2.1.6 | 53 | - |
half-life: more than 1 h | Pseudomonas sp. |
4.2.1.6 | 81 | - |
half-life: 10 min | Pseudomonas sp. |
4.2.1.6 | 95 | - |
half-life: 3 min | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.6 | 7.1 | - |
glycylglycine buffer | Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.6 | 5.9 | 8 | pH 5.9: about 40% of maximal activity, pH 8.0: about 25% of maximal activity | Pseudomonas sp. |