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Literature summary extracted from

  • Donald, A.; Sibley, D.; Lyons, D.E.; Dahms, A.S.
    D-Galactonate dehydrase. Purification and properties (1979), J. Biol. Chem., 254, 2132-2137.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.6 insensitive to repeated freeze-thaw cycles Pseudomonas sp.
4.2.1.6 stable to lyophilization in 0.05 M glycine/0.01 M sodium cacodylate, pH 8.8 Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.6 EDTA
-
 Pseudomonas sp.
4.2.1.6 iodoacetamide
-
 Pseudomonas sp.
4.2.1.6 PCMB
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.6 1.14
-
 D-galactonate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.6 Co2+ divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+ Pseudomonas sp.
4.2.1.6 Mg2+ optimal concentration: 1 mM Pseudomonas sp.
4.2.1.6 Mg2+ divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+ Pseudomonas sp.
4.2.1.6 Mn2+ divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+ Pseudomonas sp.
4.2.1.6 Ni2+ divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+ Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.6 57000
-
 4 * 57000, SDS-PAGE Pseudomonas sp.
4.2.1.6 240000 245000 gel filtration, sucrose density gradient centrifugation Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.6 D-galactonate Pseudomonas sp. growth on D-galactonate, L-arabinose and D-fucose increases the enzyme activity over the level of cells grown in nutrient broth ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.6 Pseudomonas sp.
-
 ATCC 27855
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.6
-
 Pseudomonas sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.6 D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O the immediate dehydration product is the enol which undergoes spontaneous ketonization Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.6 38
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
4.2.1.6 -20°C, 6 months, stable Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.6 D-galactonate growth on D-galactonate, L-arabinose and D-fucose increases the enzyme activity over the level of cells grown in nutrient broth Pseudomonas sp. ?
-
 ?
4.2.1.6 D-galactonate
-
 Pseudomonas sp. 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.6 tetramer 4 * 57000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.6 additional information
-
 Mg2+, dithiothreitol and D-galactonate increase the rate of thermal inactivation Pseudomonas sp.
4.2.1.6 53
-
 half-life: more than 1 h Pseudomonas sp.
4.2.1.6 81
-
 half-life: 10 min Pseudomonas sp.
4.2.1.6 95
-
 half-life: 3 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.6 7.1
-
 glycylglycine buffer Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.6 5.9 8 pH 5.9: about 40% of maximal activity, pH 8.0: about 25% of maximal activity Pseudomonas sp.