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Literature summary extracted from

  • Stadtman, T.C.; Grant, M.A.
    L-beta-Lysine mutase (Clostridium sticklandii) (1971), Methods Enzymol., 17B, 206-211.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.3.3 2'-deoxyATP or ATP required Acetoanaerobium sticklandii
5.4.3.3 2-oxobutanoate can partially replace pyruvate, which is required for reaction Acetoanaerobium sticklandii
5.4.3.3 mercaptan required Acetoanaerobium sticklandii
5.4.3.3 pyruvate required Acetoanaerobium sticklandii

General Stability

EC Number General Stability Organism
5.4.3.3 highly purified sulfhydryl-enzyme-component is relatively unstable in dilute solutions of low ionic strength. The presence of mercaptans in buffers tends to prevent aggregation. The cobamide enzyme component is stable to a variety of storage conditions even when highly purified Acetoanaerobium sticklandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.3.3 Ca2+
-
 Acetoanaerobium sticklandii
5.4.3.3 Co2+
-
 Acetoanaerobium sticklandii
5.4.3.3 iodoacetamide cobamide enzyme compound is unaffected, sulfhydryl enzyme compounds is rapidly inactivated Acetoanaerobium sticklandii
5.4.3.3 NH4+
-
 Acetoanaerobium sticklandii
5.4.3.3 Tris and analogs Acetoanaerobium sticklandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.3.3 K+ stimulates Acetoanaerobium sticklandii
5.4.3.3 Mg2+ Mg2+ or Mn2+ required Acetoanaerobium sticklandii
5.4.3.3 Mn2+ Mg2+ or Mn2+ required Acetoanaerobium sticklandii
5.4.3.3 Rb+ stimulates Acetoanaerobium sticklandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.3.3 60000
-
 x * 60000, gel filtration, sulfhydryl component, x * about approximately 150000, gel filtration, cobamide protein component Acetoanaerobium sticklandii

Organism

EC Number Organism UniProt Comment Textmining
5.4.3.3 Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.3.3
-
 Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.3.3 3,6-Diaminohexanoate r Acetoanaerobium sticklandii 3,5-Diaminohexanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.3.3 ? x * 60000, gel filtration, sulfhydryl component, x * about approximately 150000, gel filtration, cobamide protein component Acetoanaerobium sticklandii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.3.3 70
-
 10 min, sulfhydryl component is destroyed, cobamide protein component loses about 40% of its activity Acetoanaerobium sticklandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.3.3 8
-
-
 Acetoanaerobium sticklandii

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.3.3 alpha-(adenyl)-Co-5'-deoxyadenosyl cobamide tightly bound Acetoanaerobium sticklandii
5.4.3.3 ATP or 2'-deoxyATP required Acetoanaerobium sticklandii
5.4.3.3 FAD stimulates Acetoanaerobium sticklandii