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Literature summary extracted from

  • Hennig, D.; Scales, S.J.; Moreau, A.; Murley, L.L.; De Mey, J.; Kreis, T.E.
    A formiminotransferase cyclodeaminase isoform is localized to the Golgi complex and can mediate interaction of trans-Golgi network-derived vesicles with microtubules (1998), J. Biol. Chem., 273, 19602-19611.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.5 cloning and sequencing of the cDNA coding for p60, the chicken homologue of bifunctional formiminotransferase cyclodeaminase, a protein of 541 amino acids Gallus gallus
2.1.2.5 expressed in HeLa cells Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.5 cytosol
-
 Gallus gallus 5829
-
2.1.2.5 Golgi membrane localized on Golgi membranes as well as on some potentially Golgi complex-derived vesicular structures Gallus gallus 139
-
2.1.2.5 Golgi vesicle localized on Golgi membranes as well as on some potentially Golgi complex-derived vesicular structures Gallus gallus
-
-
2.1.2.5 microtubule microtubule-binding protein Gallus gallus 5874
-
4.3.1.4 cytoplasmic vesicle membrane
-
 Homo sapiens 30659
-
4.3.1.4 cytoplasmic vesicle membrane
-
 Gallus gallus 30659
-
4.3.1.4 Golgi apparatus Golgi apparatus, formiminotransferase-cyclodeaminase enzyme complex Homo sapiens 5794
-
4.3.1.4 Golgi apparatus Golgi apparatus, formiminotransferase-cyclodeaminase enzyme complex Rattus norvegicus 5794
-
4.3.1.4 Golgi apparatus Golgi apparatus, formiminotransferase-cyclodeaminase enzyme complex Gallus gallus 5794
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.5 59100
-
 x * 59100, calculated from the amino acid sequence Gallus gallus
4.3.1.4 59100
-
 8 * 59100, calculation from sequence of amono acid, isoform of formiminotransferase-cyclodeaminase enzyme complex Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Gallus gallus in addition to its enzymatic activity enzyme may have a second physiological function in mediating interaction of Golgi-derived membranes with microtubules 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Gallus gallus histidine degradation 5-formiminotetrahydrofolate + L-glutamate
-
 ?
4.3.1.4 5-formiminotetrahydrofolate Meleagris gallopavo
-
 ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate Homo sapiens
-
 ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate Rattus norvegicus
-
 ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate Canis lupus familiaris
-
 ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate Sus scrofa
-
 ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate Gallus gallus
-
 ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate Canis lupus familiaris Madin-Darby
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.5 Gallus gallus Q9YH58 chicken, several isoforms of p60, bifunctional formiminotransferase cyclodeaminase
-
2.1.2.5 Meleagris gallopavo
-
-
-
4.3.1.4 Canis lupus familiaris
-
 Madin-Darby
-
4.3.1.4 Canis lupus familiaris Madin-Darby
-
 Madin-Darby
-
4.3.1.4 Gallus gallus Q9YH58
-
-
4.3.1.4 Homo sapiens
-
-
-
4.3.1.4 Meleagris gallopavo
-
-
-
4.3.1.4 Rattus norvegicus
-
-
-
4.3.1.4 Sus scrofa P53603
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.1.2.5 additional information p60, bifunctional formiminotransferase cyclodeaminase, is post-translationally modified Gallus gallus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.5 purification from liver Meleagris gallopavo
2.1.2.5 purification from liver Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.5 hepatoma cell DU249 chicken hepatoma cells, contain p60 but no measurable formiminotransferase activity, may be due to the low expression of p60 Gallus gallus
-
2.1.2.5 liver
-
 Meleagris gallopavo
-
2.1.2.5 liver
-
 Gallus gallus
-
4.3.1.4 HeLa cell isoform of the formiminotransferase cyclodeaminase enzyme complex Homo sapiens
-
4.3.1.4 hepatoma cell
-
 Homo sapiens
-
4.3.1.4 hepatoma cell isoform of the formiminotransferase cyclodeaminase enzyme complex Gallus gallus
-
4.3.1.4 kidney isoform of the formiminotransferase cyclodeaminase enzyme complex Canis lupus familiaris
-
4.3.1.4 liver
-
 Homo sapiens
-
4.3.1.4 liver
-
 Rattus norvegicus
-
4.3.1.4 liver
-
 Sus scrofa
-
4.3.1.4 liver isoforms of the formiminotransferase cyclodeaminase enzyme complex Meleagris gallopavo
-
4.3.1.4 liver isoforms of the formiminotransferase cyclodeaminase enzyme complex Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.5 10.5
-
-
 Gallus gallus
4.3.1.4 additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.5 additional information p60: bifunctional formiminotransferase cyclodeaminase, can mediate interaction between trans-Golgi network derived vesicles and microtubule in vitro, p60 may bind to the polyglutamate on microtubules, globular hydrophilic protein Meleagris gallopavo ?
-
 ?
2.1.2.5 additional information p60: bifunctional formiminotransferase cyclodeaminase, can mediate interaction between trans-Golgi network derived vesicles and microtubule in vitro, p60 may bind to the polyglutamate on microtubules, globular hydrophilic protein Gallus gallus ?
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate in addition to its enzymatic activity enzyme may have a second physiological function in mediating interaction of Golgi-derived membranes with microtubules Gallus gallus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine degradation Gallus gallus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Meleagris gallopavo 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Gallus gallus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Meleagris gallopavo 5,10-methenyltetrahydrofolate + NH3
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Homo sapiens 5,10-methenyltetrahydrofolate + NH3
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Rattus norvegicus 5,10-methenyltetrahydrofolate + NH3
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Canis lupus familiaris 5,10-methenyltetrahydrofolate + NH3
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Sus scrofa 5,10-methenyltetrahydrofolate + NH3
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Gallus gallus 5,10-methenyltetrahydrofolate + NH3
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Canis lupus familiaris Madin-Darby 5,10-methenyltetrahydrofolate + NH3
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Meleagris gallopavo ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Homo sapiens ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Rattus norvegicus ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Canis lupus familiaris ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Sus scrofa ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Gallus gallus ?
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Canis lupus familiaris Madin-Darby ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.5 ? x * 59100, calculated from the amino acid sequence Gallus gallus
4.3.1.4 octamer
-
 Rattus norvegicus
4.3.1.4 octamer
-
 Sus scrofa
4.3.1.4 octamer 8 * 59100, calculation from sequence of amono acid, isoform of formiminotransferase-cyclodeaminase enzyme complex Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.5 additional information
-
 pI: 6.13 Gallus gallus