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Literature summary extracted from

  • Koukol, J.; Conn, E.E.
    The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare (1961), J. Biol. Chem., 236, 2692-2698.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.24 3,4-dihydroxyphenyl-DL-Ala
-
Hordeum vulgare
4.3.1.24 4-coumarate trans-p-coumarate, competitive Hordeum vulgare
4.3.1.24 4-coumarate
-
Medicago sativa
4.3.1.24 4-coumarate
-
Oryza sativa
4.3.1.24 beta-phenyl-DL-Ser
-
Hordeum vulgare
4.3.1.24 Ca2+ CaCl2, 1 mM, 57% inhibition Hordeum vulgare
4.3.1.24 Cd2+ CdCl2, 1 mM, 100% inhibition Hordeum vulgare
4.3.1.24 Cinnamic acid trans-cinnamic acid Hordeum vulgare
4.3.1.24 Cinnamic acid
-
Medicago sativa
4.3.1.24 Cinnamic acid
-
Oryza sativa
4.3.1.24 CN- KCN, 1 mM, 85% inhibition Hordeum vulgare
4.3.1.24 Cu2+ CuSO4, 1 mM, 18% inactivation Hordeum vulgare
4.3.1.24 GSH
-
Hordeum vulgare
4.3.1.24 Hg2+ HgCl2, 0.01 mM, 100% inhibition Hordeum vulgare
4.3.1.24 iodoacetamide
-
Hordeum vulgare
4.3.1.24 L-Cys
-
Hordeum vulgare
4.3.1.24 p-chloromercuriphenylsulfonic acid
-
Hordeum vulgare
4.3.1.24 Tyr competitive; L-Tyr Hordeum vulgare
4.3.1.24 Tyr
-
Medicago sativa
4.3.1.24 Zn2+ ZnSO4, 1 mM, 32% inhibition Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.24 1.7
-
L-Phe
-
Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.24 Hordeum vulgare
-
L. var. Aravat
-
4.3.1.24 Lupinus sp.
-
-
-
4.3.1.24 Medicago sativa
-
-
-
4.3.1.24 Melilotus officinalis
-
-
-
4.3.1.24 Oryza sativa
-
-
-
4.3.1.24 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.24
-
Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
4.3.1.24 -10°C, 25% loss of activity after 3.5 months Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.24 L-Phe
-
Lupinus sp. (E)-cinnamate + NH3
-
?
4.3.1.24 L-Phe
-
Pisum sativum (E)-cinnamate + NH3
-
?
4.3.1.24 L-Phe
-
Medicago sativa (E)-cinnamate + NH3
-
?
4.3.1.24 L-Phe
-
Oryza sativa (E)-cinnamate + NH3
-
?
4.3.1.24 L-Phe
-
Melilotus officinalis (E)-cinnamate + NH3
-
?
4.3.1.24 L-Phe no activity with D-Phe Hordeum vulgare (E)-cinnamate + NH3
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.24 8.8 9.2
-
Hordeum vulgare
4.3.1.24 8.8 9.2
-
Medicago sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.24 7 10.5 pH 7: about 35% of maximal activity, pH 10.5: about 70% of maximal activity Hordeum vulgare