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Literature summary extracted from
- Differential expression and properties of phenylalanine ammonia-lyase isoforms in tomato leaves (1998), Phytochemistry, 49, 2233-2243.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.24 | 0.121 | - |
L-Phe | enzyme form PAL-II | Solanum lycopersicum |  |
| 4.3.1.24 | 0.465 | - |
L-Phe | enzyme form PAL-III | Solanum lycopersicum | |
| 4.3.1.24 | 0.84 | - |
L-Phe | enzyme form PAL-I | Solanum lycopersicum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.24 | 80000 | - |
4 * 80000, SDS-PAGE | Solanum lycopersicum |
| 4.3.1.24 | 320000 | - |
gel filtration | Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.24 | additional information | Solanum lycopersicum | 3 isoforms, which are differentially induced and affected by metabolites belonging to particular branches of phenylpropanoid pathway | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.24 | Solanum lycopersicum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.1.24 | 3 isoenzymes: PAL-I, PAL-II and PAL-III | Solanum lycopersicum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.3.1.24 | leaf | - |
Solanum lycopersicum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.24 | additional information | - |
- |
Solanum lycopersicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.24 | L-Phe | - |
Solanum lycopersicum | (E)-cinnamate + NH3 | - |
? | |
| 4.3.1.24 | additional information | 3 isoforms, which are differentially induced and affected by metabolites belonging to particular branches of phenylpropanoid pathway | Solanum lycopersicum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.1.24 | tetramer | 4 * 80000, SDS-PAGE | Solanum lycopersicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.24 | 8.8 | - |
enzyme form PAL-II | Solanum lycopersicum |
| 4.3.1.24 | 9.5 | - |
enzyme form PAL-III | Solanum lycopersicum |
| 4.3.1.24 | 10.5 | - |
enzyme form PAL-I | Solanum lycopersicum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.24 | 7 | 10.5 | pH 7: about 50% of maximal activity, pH 10.5: about 70% of maximal activity, enzyme form PAL-II | Solanum lycopersicum |
| 4.3.1.24 | 7 | 10.5 | pH 7: about 30% of maximal activity, pH 10.5: about 90% of maximal activity, enzyme form PAL-III | Solanum lycopersicum |
| 4.3.1.24 | 8 | 11 | pH 8: about 50% of maximal activity, pH 11: about 80% of maximal activity, enzyme form PAL-I | Solanum lycopersicum |
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