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Literature summary extracted from

  • Tanaka, H.; Esaki, N.; Soda, K.
    Properties of L-methionine gamma-lyase from Pseudomonas ovalis (1977), Biochemistry, 16, 100-106.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.4.1.11 freezing and thawing, slight decrease in activity Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.11 carbonyl reagents
-
Pseudomonas putida
4.4.1.11 hydroxylamine
-
Pseudomonas putida
4.4.1.11 sulfhydryl inhibitors
-
Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.11 40000
-
x * 40000, x * 48000, disc gel electrophoresis, guanidine hydrochloride Pseudomonas putida
4.4.1.11 48000
-
x * 40000, x * 48000, disc gel electrophoresis, guanidine hydrochloride Pseudomonas putida
4.4.1.11 173000
-
sedimentation equilibrium method Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11
-
Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
4.4.1.11 additional information Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 ethionine
-
Pseudomonas putida ?
-
?
4.4.1.11 L-methionine + H2O
-
Pseudomonas putida methanethiol + NH3 + 2-oxobutanoate
-
ir
4.4.1.11 additional information
-
Pseudomonas putida ?
-
?
4.4.1.11 S-methyl-L-cysteine + H2O
-
Pseudomonas putida pyruvate + NH3 + CH3SH
-
ir

Subunits

EC Number Subunits Comment Organism
4.4.1.11 polymer x * 40000, x * 48000, disc gel electrophoresis, guanidine hydrochloride Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.11 50
-
pH 6.5-9.0, stable, 5 min Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.4.1.11 6.5 9 50°C, 5 min, stable in pH-range Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.11 pyridoxal 5'-phosphate 4 mol per mol of enzyme Pseudomonas putida