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Literature summary extracted from

  • Hedegaard, J.; Gunsalus, I.C.
    Mixed function oxidation. IV. An induced methylene hydroxylase in camphor oxidation (1965), J. Biol. Chem., 240, 4038-4043.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.1 tetrahydrofuran activation Pseudomonas sp.

General Stability

EC Number General Stability Organism
1.14.15.1 dialysis, loss of activity in crude extract of Pseudomonas sp. C5, restorable by NADPH-addition Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.1 cytoplasm activity sediments after 14 h centrifugation of crude extract at 140000 * g, not after 2 h at 100000 * g, Pseudomonas sp. C5 Pseudomonas sp. 5737
-
1.14.15.1 cytoplasm activity sediments after 14 h centrifugation of crude extract at 140000 * g, not after 2 h at 100000 * g, Pseudomonas sp. C5 Pseudomonas putida 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2 Pseudomonas sp.
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
1.14.15.1 (+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
1.14.15.1 (+)-camphor + putidaredoxin + O2 Pseudomonas putida C1 / ATCC 17453
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
-
-
1.14.15.1 Pseudomonas putida
-
 Accession Number PC00183
-
1.14.15.1 Pseudomonas putida C1 / ATCC 17453
-
-
-
1.14.15.1 Pseudomonas sp.
-
 soil isolate, strain C5
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.1 0.016
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.14.15.1 0-4°C, after 24-96 h, the resuspended pellet of 140000*g sedimentation of Pseudomonas sp. C5 loses hydroxylation capacity, restorable with NADPH and THF Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2
-
 Pseudomonas sp. (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
1.14.15.1 (+)-camphor + putidaredoxin + O2
-
 Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
1.14.15.1 (+)-camphor + putidaredoxin + O2
-
 Pseudomonas putida C1 / ATCC 17453 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
1.14.15.1 1,2-campholide + putidaredoxin + O2
-
 Pseudomonas sp. 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 1,2-campholide + putidaredoxin + O2
-
 Pseudomonas putida 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 1,2-campholide + putidaredoxin + O2
-
 Pseudomonas putida C1 / ATCC 17453 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 FMN
-
 Pseudomonas sp.
1.14.15.1 FMN
-
 Pseudomonas putida
1.14.15.1 NADPH
-
 Pseudomonas putida
1.14.15.1 NADPH requirement, instead of NADH, with campholide as substrate, Pseudomonas sp. C5 Pseudomonas sp.