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Literature summary extracted from

  • Koelen, K.J.; Gross, G.G.
    Partial purification and properties of tropine dehydrogenase from root cultures of Datura stramonium (1982), J. Med. Plant Res., 44, 227-230.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.206 p-hydroxymercuribenzoate 14% inhibition at 0.01 mM, 56% inhibition at 0.1 mM, 100% inhibition at 1.0 mM Datura stramonium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.206 0.013
-
 NADP+
-
 Datura stramonium
1.1.1.206 0.023
-
 NADPH
-
 Datura stramonium
1.1.1.206 0.77
-
 Tropine
-
 Datura stramonium
1.1.1.206 0.83
-
 tropinone
-
 Datura stramonium
1.1.1.206 0.91
-
 Scopine
-
 Datura stramonium
1.1.1.206 2
-
 nortropine
-
 Datura stramonium
1.1.1.206 3.03
-
 Nortropinone
-
 Datura stramonium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.206 56000
-
 gel filtration Datura stramonium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.206 tropine + NADP+ Datura stramonium last step in biosynthesis of tropine tropinone + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.206 Datura stramonium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.206 partial Datura stramonium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.206 root
-
 Datura stramonium
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.206 -18°C, 50% glycerol, 3 weeks Datura stramonium
1.1.1.206 4°C, 0.46 mg protein/ml, complete inactivation of enzyme activity over night Datura stramonium
1.1.1.206 4°C, 2.3 mg protein/ml, 2 days, complete inactivation of enzyme activity Datura stramonium
1.1.1.206 4°C, 23 mg protein/ml, 9 days, complete inactivation of enzyme activity Datura stramonium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.206 nortropine + NADP+
-
 Datura stramonium nortropinone + NADPH
-
 ?
1.1.1.206 scopine + NADP+
-
 Datura stramonium scopinone + NADPH
-
 ?
1.1.1.206 tropine + NADP+
-
 Datura stramonium tropinone + NADPH
-
 r
1.1.1.206 tropine + NADP+ last step in biosynthesis of tropine Datura stramonium tropinone + NADPH
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.206 60
-
-
 Datura stramonium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.206 50
-
-
 Datura stramonium
1.1.1.206 60
-
 thermal denaturation within 10 min Datura stramonium
1.1.1.206 70
-
 thermal denaturation within 3.5 min Datura stramonium
1.1.1.206 75
-
 immediate thermal denaturation Datura stramonium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.206 6.8
-
 tropinone reduction Datura stramonium
1.1.1.206 9.5
-
 tropine oxidation Datura stramonium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.206 6 10.5
-
 Datura stramonium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.206 NADP+
-
 Datura stramonium
1.1.1.206 NADPH
-
 Datura stramonium