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Literature summary extracted from

  • Rosenbaum, K.; Jahnke, K.; Curti, B.; Hagen, W.R.; Schnackerz, K.D.; Vanoni, M.A.
    Porcine Recombinant Dihydropyrimidine Dehydrogenase: Comparison of the spectroscopic and catalytic properties of the wild-type and C671A mutant enzymes (1998), Biochemistry, 37, 17598-17609.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.2 overproduction of wild-type and mutant enzyme in Escherichia coli Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.2 C671A 1% activity compared to that of the wild-type enzyme Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.2 NADP+
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.2 Iron 16 iron atoms per enzyme, wild-type and mutant C671A, [4Fe-4S] clusters Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.2 111000
-
 2 * 111000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.2 thymine + NADPH Sus scrofa
-
 dihydrothymine + NADP+
-
 ?
1.3.1.2 uracil + NADPH Sus scrofa rate limiting enzyme in pyrimidine degradation 5,6-dihydrouracil + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.2 wild-type and mutant enzyme Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.2 5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ comparison of substrate and cofactor binding to wild-type and mutant enzyme Sus scrofa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.2 0.0043
-
 mutant C671A Sus scrofa
1.3.1.2 0.43
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 5,6-dihydrouracil + NADP+ C671A mutant only forms complex with dihydrouracil nearly without oxidizing it Sus scrofa uracil + NADPH
-
 r
1.3.1.2 thymine + NADPH
-
 Sus scrofa dihydrothymine + NADP+
-
 ?
1.3.1.2 uracil + NADPH
-
 Sus scrofa 5,6-dihydrouracil + NADP+
-
 r
1.3.1.2 uracil + NADPH rate limiting enzyme in pyrimidine degradation Sus scrofa 5,6-dihydrouracil + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.2 dimer 2 * 111000, SDS-PAGE Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 flavin contains 2 mol FMN and 2 mol FAD per mol of enzyme, tightly associated Sus scrofa
1.3.1.2 NADPH
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.2 0.001
-
 NADP+
-
 Sus scrofa