Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lu, Z.H.; Zhang, R.; Diasio, R.B.
    Comparison of dihydropyrimidine dehydrogenase from human, rat, pig and cow liver. Biochemical and immunological properties (1993), Biochem. Pharmacol., 46, 945-952.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.2 0.0011
-
 5-fluorouracil
-
 Sus scrofa
1.3.1.2 0.0018
-
 5-fluorouracil
-
 Bos taurus
1.3.1.2 0.0024
-
 Uracil
-
 Rattus norvegicus
1.3.1.2 0.0026
-
 thymine
-
 Rattus norvegicus
1.3.1.2 0.0033
-
 5-fluorouracil
-
 Homo sapiens
1.3.1.2 0.0039
-
 Uracil
-
 Bos taurus
1.3.1.2 0.0046
-
 thymine
-
 Bos taurus
1.3.1.2 0.0048
-
 thymine
-
 Homo sapiens
1.3.1.2 0.0049
-
 Uracil
-
 Homo sapiens
1.3.1.2 0.0059
-
 Uracil
-
 Sus scrofa
1.3.1.2 0.0068
-
 thymine
-
 Sus scrofa
1.3.1.2 0.0082
-
 NADPH in presence of 5-fluorouracil Sus scrofa
1.3.1.2 0.0085
-
 NADPH in presence of 5-fluorouracil Bos taurus
1.3.1.2 0.0089
-
 5-fluorouracil
-
 Rattus norvegicus
1.3.1.2 0.0089
-
 NADPH in presence of uracil Bos taurus
1.3.1.2 0.0096
-
 NADPH in presence of uracil Homo sapiens
1.3.1.2 0.01
-
 NADPH in presence of 5-fluorouracil Homo sapiens
1.3.1.2 0.0119
-
 NADPH in presence of uracil Sus scrofa
1.3.1.2 0.0158
-
 NADPH in presence of thymine Homo sapiens
1.3.1.2 0.0167
-
 NADPH in presence of thymine Sus scrofa
1.3.1.2 0.0206
-
 NADPH in presence of uracil Rattus norvegicus
1.3.1.2 0.0209
-
 NADPH in presence of 5-fluorouracil Rattus norvegicus
1.3.1.2 0.0289
-
 NADPH in presence of thymine Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.2 cytoplasm
-
 Homo sapiens 5737
-
1.3.1.2 cytoplasm
-
 Rattus norvegicus 5737
-
1.3.1.2 cytoplasm
-
 Sus scrofa 5737
-
1.3.1.2 cytoplasm
-
 Bos taurus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.2 102000
-
 2 * 102000, SDS-PAGE Sus scrofa
1.3.1.2 105000
-
 2 * 105000, SDS-PAGE Homo sapiens
1.3.1.2 105000
-
 2 * 105000, SDS-PAGE Rattus norvegicus
1.3.1.2 108000
-
 2 * 108000, SDS-PAGE Bos taurus
1.3.1.2 204000
-
 HPLC gel filtration Sus scrofa
1.3.1.2 210000
-
 gel filtration Homo sapiens
1.3.1.2 210000
-
 gel filtration Rattus norvegicus
1.3.1.2 216000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.2 thymine + NADPH Rattus norvegicus
-
 dihydrothymine + NADP+
-
 ?
1.3.1.2 thymine + NADPH Sus scrofa
-
 dihydrothymine + NADP+
-
 ?
1.3.1.2 thymine + NADPH Homo sapiens rate-limiting enzyme in catabolic detoxification of exogenous fluoropyrimidines dihydrothymine + NADP+
-
 ?
1.3.1.2 thymine + NADPH Bos taurus rate-limiting enzyme in catabolic detoxification of exogenous fluoropyrimidines dihydrothymine + NADP+
-
 ?
1.3.1.2 uracil + NADPH Homo sapiens rate limiting enzyme in pyrimidine degradation 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH Rattus norvegicus rate limiting enzyme in pyrimidine degradation 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH Sus scrofa rate limiting enzyme in pyrimidine degradation 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH Bos taurus rate limiting enzyme in pyrimidine degradation 5,6-dihydrouracil + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Bos taurus
-
-
-
1.3.1.2 Homo sapiens
-
-
-
1.3.1.2 Rattus norvegicus
-
-
-
1.3.1.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.2
-
 Homo sapiens
1.3.1.2
-
 Rattus norvegicus
1.3.1.2
-
 Sus scrofa
1.3.1.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.2 liver
-
 Homo sapiens
-
1.3.1.2 liver
-
 Rattus norvegicus
-
1.3.1.2 liver
-
 Sus scrofa
-
1.3.1.2 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 5-fluorouracil + NADPH + H+
-
 Homo sapiens 5-fluoro-5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 5-fluorouracil + NADPH + H+
-
 Rattus norvegicus 5-fluoro-5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 5-fluorouracil + NADPH + H+
-
 Sus scrofa 5-fluoro-5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 5-fluorouracil + NADPH + H+
-
 Bos taurus 5-fluoro-5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 dihydrothymine + NADP+
-
 Homo sapiens thymine + NADPH
-
 r
1.3.1.2 dihydrothymine + NADP+
-
 Rattus norvegicus thymine + NADPH
-
 r
1.3.1.2 dihydrothymine + NADP+
-
 Sus scrofa thymine + NADPH
-
 r
1.3.1.2 dihydrothymine + NADP+
-
 Bos taurus thymine + NADPH
-
 r
1.3.1.2 thymine + NADPH
-
 Homo sapiens dihydrothymine + NADP+
-
 ?
1.3.1.2 thymine + NADPH
-
 Rattus norvegicus dihydrothymine + NADP+
-
 ?
1.3.1.2 thymine + NADPH
-
 Sus scrofa dihydrothymine + NADP+
-
 ?
1.3.1.2 thymine + NADPH
-
 Bos taurus dihydrothymine + NADP+
-
 ?
1.3.1.2 thymine + NADPH rate-limiting enzyme in catabolic detoxification of exogenous fluoropyrimidines Homo sapiens dihydrothymine + NADP+
-
 ?
1.3.1.2 thymine + NADPH rate-limiting enzyme in catabolic detoxification of exogenous fluoropyrimidines Bos taurus dihydrothymine + NADP+
-
 ?
1.3.1.2 uracil + NADPH
-
 Homo sapiens 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH
-
 Rattus norvegicus 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH
-
 Sus scrofa 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH
-
 Bos taurus 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH rate limiting enzyme in pyrimidine degradation Homo sapiens 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH rate limiting enzyme in pyrimidine degradation Rattus norvegicus 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH rate limiting enzyme in pyrimidine degradation Sus scrofa 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH rate limiting enzyme in pyrimidine degradation Bos taurus 5,6-dihydrouracil + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.2 dimer 2 * 105000, SDS-PAGE Homo sapiens
1.3.1.2 dimer 2 * 105000, SDS-PAGE Rattus norvegicus
1.3.1.2 dimer 2 * 108000, SDS-PAGE Bos taurus
1.3.1.2 dimer 2 * 102000, SDS-PAGE Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 NADPH
-
 Homo sapiens
1.3.1.2 NADPH
-
 Rattus norvegicus
1.3.1.2 NADPH
-
 Sus scrofa
1.3.1.2 NADPH
-
 Bos taurus