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Literature summary extracted from

  • Caballero, F.J.; Cardenas, J.; Castillo, F.
    Purification and properties of L-alanine dehydrogenase of the phototrophic bacterium Rhodobacter capsulatus E1F1 (1989), J. Bacteriol., 171, 3205-3210.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.1 2-mercaptoethanol activation Rhodobacter capsulatus
1.4.1.1 Cys activates Rhodobacter capsulatus
1.4.1.1 dithioerythritol activates Rhodobacter capsulatus
1.4.1.1 dithiothreitol activation Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 L-Ala 5 mM, 34% inhibition Rhodobacter capsulatus
1.4.1.1 L-Ser 10 mM, 26% inhibition; inhibition of aminating activity Rhodobacter capsulatus
1.4.1.1 p-hydroxymercuribenzoate no effect on aminating activity, inhibition of deaminating activity Rhodobacter capsulatus
1.4.1.1 pyruvate product inhibition Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.13
-
 pyruvate
-
 Rhodobacter capsulatus
1.4.1.1 0.15
-
 NAD+
-
 Rhodobacter capsulatus
1.4.1.1 0.25
-
 NADH
-
 Rhodobacter capsulatus
1.4.1.1 1.25
-
 L-alanine
-
 Rhodobacter capsulatus
1.4.1.1 16
-
 NH4+
-
 Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 42000
-
 6 * 42000, SDS-PAGE Rhodobacter capsulatus
1.4.1.1 240000 241000 gel filtration Rhodobacter capsulatus
1.4.1.1 246000
-
 non-denaturing PAGE Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+ Rhodobacter capsulatus alternative route for ammonia assimilation when glutamine synthetase is inactivated pyruvate + NH3 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Rhodobacter capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Rhodobacter capsulatus

Storage Stability

EC Number Storage Stability Organism
1.4.1.1 4°C, stable Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
 Rhodobacter capsulatus pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+ alternative route for ammonia assimilation when glutamine synthetase is inactivated Rhodobacter capsulatus pyruvate + NH3 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.1 hexamer 6 * 42000, SDS-PAGE Rhodobacter capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 27
-
 deaminating activity Rhodobacter capsulatus
1.4.1.1 30
-
 aminating activity Rhodobacter capsulatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 50
-
 50% loss of aminating activity after 20 min, 50% loss of deaminating activity after 27 min Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8.2
-
 amination of pyruvate Rhodobacter capsulatus
1.4.1.1 10.5
-
 deamination of L-Ala Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+ specific for Rhodobacter capsulatus
1.4.1.1 NADH
-
 Rhodobacter capsulatus
1.4.1.1 NADPH aminating activity absolutely specific for Rhodobacter capsulatus