Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Misono, H.; Yonezawa, J.; Nagata, S.; Nagasaki, S.
    Purification and characterization of a dimeric phenylalanine dehydrogenase from Rhodococcus maris K-18 (1989), J. Bacteriol., 171, 30-36.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.4.1.20 20% glycerol, 0.25 M sodium malonate or 0.5 M sodium glutarate stabilize the enzyme during storage at 4°C Dietzia maris
1.4.1.20 unstable in absence of glycerol and salt Dietzia maris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.20 3-Phenylpropionate competitive with L-Phe Dietzia maris
1.4.1.20 4-hydroxyphenylethylamine competitive with L-Phe Dietzia maris
1.4.1.20 alpha-methyl-DL-phenylalanine
-
 Dietzia maris
1.4.1.20 Cu2+ slight Dietzia maris
1.4.1.20 D-ethionine
-
 Dietzia maris
1.4.1.20 D-Phe competitive with L-Phe Dietzia maris
1.4.1.20 D-Tyr competitive with L-Phe Dietzia maris
1.4.1.20 HgCl2 0.01 mM, complete inhibition Dietzia maris
1.4.1.20 L-Phe product inhibition Dietzia maris
1.4.1.20 L-phenylglycine competitive with L-Phe Dietzia maris
1.4.1.20 NAD+ product inhibition Dietzia maris
1.4.1.20 PCMB 0.001 mM, complete inhibition Dietzia maris
1.4.1.20 phenylenediamine
-
 Dietzia maris
1.4.1.20 Phenylethylamine competitive with L-Phe Dietzia maris
1.4.1.20 trans-cinnamate competitive with L-Phe Dietzia maris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.20 0.18
-
 deamino-NAD+
-
 Dietzia maris
1.4.1.20 0.18
-
 thionicotinamide-NAD+
-
 Dietzia maris
1.4.1.20 0.25
-
 NAD+
-
 Dietzia maris
1.4.1.20 0.25
-
 3-acetylpyridine-NAD+
-
 Dietzia maris
1.4.1.20 0.5
-
 phenylpyruvate
-
 Dietzia maris
1.4.1.20 1.3
-
 4-hydroxyphenylpyruvate
-
 Dietzia maris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.20 36000
-
 2 * 36000, SDS-PAGE Dietzia maris
1.4.1.20 70000
-
 gel filtration Dietzia maris

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Dietzia maris
-
 K-18
-
1.4.1.20 Dietzia maris K-18
-
 K-18
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.20
-
 Dietzia maris

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.1.20 L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH + H+ the reductive amination proceeds through a sequential ordered ternary-binary mechanism Dietzia maris
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.20 65.2
-
-
 Dietzia maris

Storage Stability

EC Number Storage Stability Organism
1.4.1.20 4°C, 0.25 M sodium malonate, stable for 1 month Dietzia maris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 4-hydroxyphenylpyruvate + NH3 + NADH + H+
-
 Dietzia maris L-tyrosine + NAD+ + H2O
-
 r
1.4.1.20 L-alpha-amino-beta-phenylbutyrate + H2O + NAD+ 7.0% of the activity with L-Phe Dietzia maris 2-oxo-3-phenylbutyrate + NADH + NH3
-
 ?
1.4.1.20 L-ethionine + H2O + NAD+ 13.0% of the activity with L-Phe Dietzia maris 4-ethylthio-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.20 L-norleucine + H2O + NAD+ 15.6% of the activity with L-Phe Dietzia maris 2-oxohexanoic acid + NADH + NH3
-
 ?
1.4.1.20 L-Phe + H2O + NAD+ the reductive amination proceeds through a sequential ordered ternary-binary mechanism Dietzia maris phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-Phe + H2O + NAD+ the reductive amination proceeds through a sequential ordered ternary-binary mechanism Dietzia maris K-18 phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-phenylalanine + H2O + 3-acetylpyridine-NAD+ at 241% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + acetylpyridine-NADH
-
 ?
1.4.1.20 L-phenylalanine + H2O + 3-acetylpyridine-NAD+ at 241% of the activity with NAD+ Dietzia maris K-18 phenylpyruvate + NH3 + acetylpyridine-NADH
-
 ?
1.4.1.20 L-phenylalanine + H2O + 3-pyridinealdehyde-NAD+ at 9.2% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + 3-pyridinealdehyde-NADH
-
 ?
1.4.1.20 L-phenylalanine + H2O + deamino-NAD+ at 86% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + deamino-NADH
-
 ?
1.4.1.20 L-phenylalanine + H2O + deamino-NAD+ at 86% of the activity with NAD+ Dietzia maris K-18 phenylpyruvate + NH3 + deamino-NADH
-
 ?
1.4.1.20 L-phenylalanine + H2O + NADP+ at 6.6% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + NADPH
-
 ?
1.4.1.20 L-phenylalanine + H2O + NADP+ at 6.6% of the activity with NAD+ Dietzia maris K-18 phenylpyruvate + NH3 + NADPH
-
 ?
1.4.1.20 L-phenylalanine + H2O + thionicotinamide-NAD+ at 101% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + thionicotinamide-NADH
-
 ?
1.4.1.20 L-phenylalanine + H2O + thionicotinamide-NAD+ at 101% of the activity with NAD+ Dietzia maris K-18 phenylpyruvate + NH3 + thionicotinamide-NADH
-
 ?
1.4.1.20 L-Trp + H2O + NAD+ 7.5% of the activity with L-Phe Dietzia maris indole-3-pyruvate + NH3 + NADH
-
 ?
1.4.1.20 m-fluoro-DL-phenylalanine + H2O + NAD+ 7.7% of the activity with L-Phe Dietzia maris 3-(3-fluorophenyl)-2-oxopropionate + NADH + NH3
-
 ?
1.4.1.20 p-fluoro-DL-phenylalanine + H2O + NAD+ 8.3% of the activity with L-Phe Dietzia maris (4-fluorophenyl)-2-oxopropionate + NADH + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.20 dimer 2 * 36000, SDS-PAGE Dietzia maris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.20 9.8
-
 reductive amination of phenylpyruvate Dietzia maris
1.4.1.20 10.8
-
 oxidative deamination of L-Phe Dietzia maris

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 3-acetylpyridine-NAD+ 241% of the activity with NAD+ in the reaction with L-Phe Dietzia maris
1.4.1.20 3-pyridinealdehyde-NAD+ 9.2% of the activity with NAD+ in the reaction with L-Phe Dietzia maris
1.4.1.20 deamino-NAD+ 86% of the activity with NAD+ in the reaction with L-Phe Dietzia maris
1.4.1.20 NAD+
-
 Dietzia maris
1.4.1.20 NADH
-
 Dietzia maris
1.4.1.20 thionicotinamide-NAD+ 101% of the activity with NAD+ in the reaction with L-Phe Dietzia maris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.20 0.14
-
 D-Phe
-
 Dietzia maris
1.4.1.20 1.52
-
 Phenylethylamine
-
 Dietzia maris
1.4.1.20 1.58
-
 D-Tyr
-
 Dietzia maris
1.4.1.20 1.76
-
 D-ethionine
-
 Dietzia maris
1.4.1.20 2.6
-
 3-Phenylpropionate
-
 Dietzia maris
1.4.1.20 4.36
-
 phenylenediamine
-
 Dietzia maris
1.4.1.20 8.32
-
 L-phenylglycine
-
 Dietzia maris
1.4.1.20 12.7
-
 trans-cinnamic acid
-
 Dietzia maris
1.4.1.20 13.7
-
 4-hydroxyphenylethylamine
-
 Dietzia maris
1.4.1.20 20.8
-
 alpha-methyl-DL-phenylalanine
-
 Dietzia maris