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Literature summary extracted from
- Reductive dechlorination of tetrachloroethene to ethene by a two-component enzyme pathway (1998), Appl. Environ. Microbiol., 64, 1270-1275.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.21.99.5 | agriculture | degradation of lipophilic solvent tetrachloroethene, one of the most abundant halogenated xenobiotic pollutants in the environment | Dehalococcoides mccartyi |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.21.99.5 | cysteine stabilizes | Dehalococcoides mccartyi |
| 1.21.99.5 | enzyme very labile during purification | Dehalococcoides mccartyi |
| 1.21.99.5 | ferrous ammonium sulfate stabilizes | Dehalococcoides mccartyi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | Cu2+ | - |
Dehalococcoides mccartyi |  |
| 1.21.99.5 | CuCl2 | 1 mM cuprous chloride: slight inhibition | Dehalococcoides mccartyi | |
| 1.21.99.5 | cyanide | 20 mM sodium cyanide: 20% inhibition | Dehalococcoides mccartyi | |
| 1.21.99.5 | Dithionite | 2 mM sodium dithionite: complete inhibition | Dehalococcoides mccartyi | |
| 1.21.99.5 | EDTA | no inhibition by 5 mM EDTA | Dehalococcoides mccartyi | |
| 1.21.99.5 | Iodoethane | in the presence of titanium(III)citrate: 85% inhibition; light-reversible inhibition | Dehalococcoides mccartyi | |
| 1.21.99.5 | additional information | no inhibition by 2 mM sodium sulfate, sodium selenate, sodium sulfide, or 100% carbon monoxide; no inhibition by 5 mM EDTA, bathophenanthroline disulfonate, and 2,2-dipyridyl | Dehalococcoides mccartyi | |
| 1.21.99.5 | Propyl iodide | no inhibition in the presence of 1-iodopropane and titanium(III)citrate | Dehalococcoides mccartyi | |
| 1.21.99.5 | sulfite | 2 mM sodium sulfite: complete inhibition | Dehalococcoides mccartyi | |
| 1.21.99.5 | Zn2+ | - |
Dehalococcoides mccartyi | |
| 1.21.99.5 | ZnCl2 | 5 mM zinc cloride: slight inhibition | Dehalococcoides mccartyi | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.21.99.5 | cytoplasmic membrane | membrane-bound | Dehalococcoides mccartyi | - |
- |
| 1.21.99.5 | cytoplasmic membrane | cell wall-membrane | Dehalococcoides mccartyi | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | Cobalt | Co(I) corrinoid cofactor | Dehalococcoides mccartyi | |
| 1.21.99.5 | Fe2+ | iron-sulfur cluster | Dehalococcoides mccartyi | |
| 1.21.99.5 | Iron-sulfur cluster | - |
Dehalococcoides mccartyi | |
| 1.21.99.5 | iron-sulfur clusters | - |
Dehalococcoides mccartyi | |
| 1.21.99.5 | S | iron-sulfur cluster | Dehalococcoides mccartyi | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 51000 | - |
PAGE | Dehalococcoides mccartyi |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | tetrachloroethene + reduced acceptor | Dehalococcoides mccartyi | - |
trichloroethene + chloride + acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.21.99.5 | Dehalococcoides mccartyi | - |
195 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.21.99.5 | partial | Dehalococcoides mccartyi |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.21.99.5 | trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | mechanism | Dehalococcoides mccartyi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 20.5 | - |
- |
Dehalococcoides mccartyi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | additional information | substrate specificity | Dehalococcoides mccartyi | ? | - |
? | |
| 1.21.99.5 | additional information | no activity with trichloroethene, TCE, cis-dichloroethene, cis-DCE, or vinyl chloride | Dehalococcoides mccartyi | ? | - |
? | |
| 1.21.99.5 | additional information | enzyme quite specific | Dehalococcoides mccartyi | ? | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | - |
Dehalococcoides mccartyi | trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | quite specific for tetrachloroethene | Dehalococcoides mccartyi | trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Dehalococcoides mccartyi | trichloroethene + chloride + acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.21.99.5 | monomer | 1 * 51000, SDS-PAGE | Dehalococcoides mccartyi |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | additional information | - |
stable at neutral pH | Dehalococcoides mccartyi |
| 1.21.99.5 | 5 | 8 | unstable above pH 8 or below pH 5 | Dehalococcoides mccartyi |
| 1.21.99.5 | 7 | - |
stable at neutral pH | Dehalococcoides mccartyi |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | corrinoid | Co(I) corrinoid cofactor | Dehalococcoides mccartyi | |
| 1.21.99.5 | vitamin B12 | - |
Dehalococcoides mccartyi | |
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Release 2023.1 (January 2023)
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