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Literature summary extracted from

  • Clark, D.D.; Ensign, S.A.
    Evidence for an inducible nucleotide-dependent acetone carboxylase in Rhodococcus rhodochrous B276 (1999), J. Bacteriol., 181, 2752-2758.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.6 additional information no inhibition by avidin Rhodococcus rhodochrous
6.4.1.6 additional information
-
Xanthobacter autotrophicus Py2

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.6 K+ required as stimulatory but not essential cofactor Xanthobacter autotrophicus Py2
6.4.1.6 Mg2+
-
Rhodobacter capsulatus
6.4.1.6 Mg2+ required as stimulatory but not essential cofactor Xanthobacter autotrophicus Py2
6.4.1.6 Mg2+ stimulatory effect on enzyme activity Rhodococcus rhodochrous
6.4.1.6 additional information
-
Xanthobacter autotrophicus Py2
6.4.1.6 additional information dependent on the presence of a divalent metal Rhodococcus rhodochrous
6.4.1.6 NH4+ stimulatory effect on enzyme activity Rhodococcus rhodochrous

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.6 16000
-
1 * 85000, 1 * 74000, 1 * 16000, SDS-PAGE Rhodococcus rhodochrous
6.4.1.6 19600
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 23000
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 68400
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 74000
-
1 * 85000, 1 * 74000, 1 * 16000, SDS-PAGE Rhodococcus rhodochrous
6.4.1.6 78300
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 78800
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 85000
-
1 * 85000, 1 * 74000, 1 * 16000, SDS-PAGE Rhodococcus rhodochrous
6.4.1.6 85300
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.6 Rhodobacter capsulatus
-
-
-
6.4.1.6 Rhodococcus rhodochrous
-
B276, ATCC 31338, formerly Nocardia corallina
-
6.4.1.6 Xanthobacter autotrophicus Py2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.6 DEAE-Sepharose chromatography, but hydrophobic interaction, gel filtration and anion-exchange chromatography by Q-Sepharose resulted in large loss of activity Rhodococcus rhodochrous

Reaction

EC Number Reaction Comment Organism Reaction ID
6.4.1.6 acetone + CO2 + ATP + 2 H2O = acetoacetate + AMP + 2 phosphate activity is induced in suspensions of acetone- and isopropanol-grown cells Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.6 additional information
-
-
Rhodobacter capsulatus
6.4.1.6 additional information
-
GTP shows best specific activity of tested nucleoside triphosphates: ATP, ITP, XTP, CTP and UTP Rhodococcus rhodochrous
6.4.1.6 0.0015 0.003 cell extracts Rhodococcus rhodochrous
6.4.1.6 0.014
-
with ITP as nucleoside triphosphate Rhodococcus rhodochrous
6.4.1.6 0.016
-
with GTP as nucleoside triphosphate Rhodococcus rhodochrous
6.4.1.6 0.07 0.15 cell suspension Rhodococcus rhodochrous
6.4.1.6 0.225
-
-
Xanthobacter autotrophicus Py2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.6 acetone + CO2 + ATP + H2O
-
Rhodobacter capsulatus acetoacetate + AMP + phosphate
-
?
6.4.1.6 acetone + CO2 + ATP + H2O
-
Xanthobacter autotrophicus Py2 acetoacetate + AMP + phosphate
-
?
6.4.1.6 acetone + CO2 + ATP + H2O low activity with ATP Rhodococcus rhodochrous acetoacetate + AMP + phosphate
-
?
6.4.1.6 acetone + CO2 + GTP + H2O better activity than with ATP Rhodococcus rhodochrous acetoacetate + GMP + phosphate
-
?
6.4.1.6 butanone + CO2 + ATP + H2O
-
Rhodococcus rhodochrous 3-oxopentanoate + AMP + phosphate
-
?
6.4.1.6 butanone + CO2 + ATP + H2O
-
Rhodobacter capsulatus 3-oxopentanoate + AMP + phosphate
-
?
6.4.1.6 butanone + CO2 + ATP + H2O
-
Xanthobacter autotrophicus Py2 3-oxopentanoate + AMP + phosphate
-
?
6.4.1.6 additional information enzyme expressed by acetone and isopropanol grown cells, not by propylene or glucose grown cells Rhodococcus rhodochrous ?
-
?
6.4.1.6 additional information enzyme expressed by acetone and isopropanol grown cells, not by propylene or glucose grown cells Xanthobacter autotrophicus Py2 ?
-
?
6.4.1.6 additional information 2-pentanone, 3-pentanone and 2-hexanone as well as short-chain aliphatic alkanes and alkenes, including propane, are substrates. Several other nucleoside triphosphates are used Rhodococcus rhodochrous ?
-
?

Subunits

EC Number Subunits Comment Organism
6.4.1.6 trimer 1 * 85000, 1 * 74000, 1 * 16000, SDS-PAGE Rhodococcus rhodochrous
6.4.1.6 trimer 1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.6 30
-
-
Rhodococcus rhodochrous
6.4.1.6 30
-
-
Xanthobacter autotrophicus Py2
6.4.1.6 30
-
assay at Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.6 8
-
assay at Rhodococcus rhodochrous

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.6 ATP
-
Rhodobacter capsulatus
6.4.1.6 ATP
-
Xanthobacter autotrophicus Py2
6.4.1.6 ATP low activity Rhodococcus rhodochrous
6.4.1.6 GTP
-
Rhodococcus rhodochrous
6.4.1.6 ITP
-
Rhodococcus rhodochrous
6.4.1.6 additional information nucleoside triphosphate required, ATP does not support acetone carboxylation Rhodococcus rhodochrous