EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.4 | sulfhydryl groups | contains active thiol groups | Eubacterium barkeri |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.4 | 1-Methyl-1,2-cis-cyclopropanedicarboxylate | noncompetitive, slight inhibitor | Eubacterium barkeri | |
5.4.99.4 | 1-Methyl-1,2-trans-cyclopropanedicarboxylate | competitive | Eubacterium barkeri | |
5.4.99.4 | citrate | slight | Eubacterium barkeri | |
5.4.99.4 | glutaconate | noncompetitive | Eubacterium barkeri | |
5.4.99.4 | iodoacetamide | - |
Eubacterium barkeri | |
5.4.99.4 | Itaconate | competitive | Eubacterium barkeri | |
5.4.99.4 | L-malate | competitive | Eubacterium barkeri | |
5.4.99.4 | Maleate | competitive | Eubacterium barkeri | |
5.4.99.4 | succinate | - |
Eubacterium barkeri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.99.4 | 70000 | - |
4 * 70000, SDS-PAGE | Eubacterium barkeri |
5.4.99.4 | 300000 | - |
gel filtration, sucrose density gradient centrifugation, nondenaturing PAGE | Eubacterium barkeri |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.4 | additional information | Eubacterium barkeri | carbon-skeleton rearrangement mechanism. Adenosylcobalamin acts as a carrier for the hydrogen atom that is abstracted from alpha-methyleneglutarate and replaced by the acrylyl group of 2-methyleneglutarate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.4 | Eubacterium barkeri | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
5.4.99.4 | enzyme containing cobalamin(II) is stable to oxygen and is an inactive form of the enzyme | Eubacterium barkeri |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.4 | - |
Eubacterium barkeri |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.4.99.4 | 2-Methyleneglutarate = 2-methylene-3-methylsuccinate | mechanism | Eubacterium barkeri | |
5.4.99.4 | 2-Methyleneglutarate = 2-methylene-3-methylsuccinate | the steric course of the reaction is such that the acrylyl residue migrates from the beta-carbon to the alpha-carbon of the propionate with inversion of configuration at the alpha-carbon | Eubacterium barkeri | |
5.4.99.4 | 2-Methyleneglutarate = 2-methylene-3-methylsuccinate | unimolecular free radical rearrangement via 3-butenyl and cyclopropylmethyl intermediates is a reasonable mechanism for the rearrangement step of the enzyme | Eubacterium barkeri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.4 | 2-Methyleneglutarate | r | Eubacterium barkeri | 2-Methylene-3-methylsuccinate | - |
? | |
5.4.99.4 | additional information | carbon-skeleton rearrangement mechanism. Adenosylcobalamin acts as a carrier for the hydrogen atom that is abstracted from alpha-methyleneglutarate and replaced by the acrylyl group of 2-methyleneglutarate | Eubacterium barkeri | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.99.4 | tetramer | 4 * 70000, SDS-PAGE | Eubacterium barkeri |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.4 | cobamide | acts as a carrier for the hydrogen atom that is abstracted from alpha-methyleneglutarate. The enzyme contains 4 cobalamin molecules per tetramer. The cobalamins consist of adenosylcobalamin and cobalamin(II). Enzyme containing adenosylcobalamin is the active form of the enzyme but the enzyme containing cobalamin(II) is stable to oxygen and is an inactive form of the enzyme | Eubacterium barkeri |