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Literature summary extracted from

  • Ahmad, S.; Wilson, A.T.; Jensen, R.A.
    Chorismate mutase: prephenate dehydratase from Acinetobacter calcoaceticus. Purification, properties and immunological cross-reactivity (1988), Eur. J. Biochem., 176, 69-79.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.51 L-tyrosine 0.1 mM, 24fold activation Acinetobacter calcoaceticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.51 L-phenylalanine
-
Acinetobacter calcoaceticus
4.2.1.51 m-Fluorophenylalanine
-
Acinetobacter calcoaceticus
4.2.1.51 p-Fluorophenylalanine
-
Acinetobacter calcoaceticus
5.4.99.5 L-Phe poor noncompetitive Acinetobacter calcoaceticus
5.4.99.5 prephenate competitive Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.55
-
chorismate
-
Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.51 potassium phosphate 1.4-2 M, activation Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.51 91000
-
bifunctional enzyme, chorismate mutase:prephenate dehydratase, absence of L-Phe, gel filtration Acinetobacter calcoaceticus
4.2.1.51 175000
-
bifunctional enzyme, chorismate mutase:prephenate dehydratase, presence of L-Phe, gel filtration Acinetobacter calcoaceticus
5.4.99.5 45000
-
2 * 45000, SDS-PAGE Acinetobacter calcoaceticus
5.4.99.5 91000
-
gel filtration, in presence of Phe or Tyr a MW of 175000 is measured Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.51 prephenate Acinetobacter calcoaceticus
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.51 Acinetobacter calcoaceticus
-
-
-
5.4.99.5 Acinetobacter calcoaceticus
-
bifunctional enzyme: chorismate mutase/prephenate dehydratase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.51 bifunctional enzyme, chorismate mutase:prephenate dehydratase Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.51 84.2
-
-
Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.51 prephenate
-
Acinetobacter calcoaceticus phenylpyruvate + H2O + CO2
-
?
4.2.1.51 prephenate
-
Acinetobacter calcoaceticus ?
-
?
5.4.99.5 Chorismate
-
Acinetobacter calcoaceticus Prephenate
-
?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 dimer 2 * 45000, SDS-PAGE Acinetobacter calcoaceticus