EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.51 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli | |
4.2.1.51 | L-phenylalanine | one phenylalanine-binding site per subunit | Escherichia coli | |
4.2.1.51 | N-ethylmaleimide | - |
Escherichia coli | |
4.2.1.51 | tetrathionate | - |
Escherichia coli | |
5.4.99.5 | L-Phe | - |
Escherichia coli | |
5.4.99.5 | L-Phe | - |
Klebsiella aerogenes | |
5.4.99.5 | prephenate | competitive | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.51 | 0.47 | 1 | prephenate | - |
Escherichia coli | |
5.4.99.5 | additional information | - |
additional information | when the pH increases from pH 6.2 to pH 8.6 the Km-values for prephenate and chorismate increase substantially | Escherichia coli | |
5.4.99.5 | 0.47 | 1 | prephenate | - |
Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.51 | 43111 | - |
2 * 43111, smallest native species of enzyme, calculation from nucleotide sequence | Escherichia coli |
4.2.1.51 | 43111 | - |
8 * 43111, calculation from nucleotide sequence | Escherichia coli |
5.4.99.5 | 40000 | - |
x * 40000, the smallest native species of the enzyme, determined by sedimentation equilibrium, appears to be a dimer | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.51 | prephenate | Escherichia coli | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.51 | Escherichia coli | - |
- |
- |
5.4.99.5 | Cupriavidus necator | - |
basonym Alcaligenes eutrophus | - |
5.4.99.5 | Escherichia coli | - |
K12 | - |
5.4.99.5 | Escherichia coli | - |
bifunctional enzyme chorismate mutase/prephenate dehydratase | - |
5.4.99.5 | Klebsiella aerogenes | - |
- |
- |
5.4.99.5 | Pseudomonas sp. | - |
- |
- |
5.4.99.5 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
5.4.99.5 | Xanthomonas campestris | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.51 | bifunctional enzyme, chorismate mutase:prephenate dehydratase | Escherichia coli |
5.4.99.5 | chorismate mutase/prephenate dehydratase | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.51 | 169 | - |
- |
Escherichia coli |
5.4.99.5 | additional information | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.51 | 4°C, 20 mM Tris-HCl, pH 8.2, 1 mM EDTA, 10 mM 2-mercaptoethanol, 0.3 M NaCl, 0.02% w/v NaN3, 1 mM dihiothreitol, 2 weeks stable | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.51 | prephenate | - |
Escherichia coli | phenylpyruvate + H2O + CO2 | - |
? | |
4.2.1.51 | prephenate | - |
Escherichia coli | ? | - |
? | |
5.4.99.5 | Chorismate | - |
Escherichia coli | Prephenate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.51 | dimer | 2 * 43111, smallest native species of enzyme, calculation from nucleotide sequence | Escherichia coli |
4.2.1.51 | octamer | 8 * 43111, calculation from nucleotide sequence | Escherichia coli |
5.4.99.5 | ? | x * 40000, the smallest native species of the enzyme, determined by sedimentation equilibrium, appears to be a dimer | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.51 | 6.2 | 8.6 | - |
Escherichia coli |