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Literature summary extracted from

  • Davidson, B.E.
    Chorismate mutase-prephenate dehydratase from Escherichia coli (1987), Methods Enzymol., 142, 432-439.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.51 5,5'-dithiobis(2-nitrobenzoate)
-
Escherichia coli
4.2.1.51 L-phenylalanine one phenylalanine-binding site per subunit Escherichia coli
4.2.1.51 N-ethylmaleimide
-
Escherichia coli
4.2.1.51 tetrathionate
-
Escherichia coli
5.4.99.5 L-Phe
-
Escherichia coli
5.4.99.5 L-Phe
-
Klebsiella aerogenes
5.4.99.5 prephenate competitive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.51 0.47 1 prephenate
-
Escherichia coli
5.4.99.5 additional information
-
additional information when the pH increases from pH 6.2 to pH 8.6 the Km-values for prephenate and chorismate increase substantially Escherichia coli
5.4.99.5 0.47 1 prephenate
-
Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.51 43111
-
2 * 43111, smallest native species of enzyme, calculation from nucleotide sequence Escherichia coli
4.2.1.51 43111
-
8 * 43111, calculation from nucleotide sequence Escherichia coli
5.4.99.5 40000
-
x * 40000, the smallest native species of the enzyme, determined by sedimentation equilibrium, appears to be a dimer Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.51 prephenate Escherichia coli
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.51 Escherichia coli
-
-
-
5.4.99.5 Cupriavidus necator
-
basonym Alcaligenes eutrophus
-
5.4.99.5 Escherichia coli
-
K12
-
5.4.99.5 Escherichia coli
-
bifunctional enzyme chorismate mutase/prephenate dehydratase
-
5.4.99.5 Klebsiella aerogenes
-
-
-
5.4.99.5 Pseudomonas sp.
-
-
-
5.4.99.5 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
5.4.99.5 Xanthomonas campestris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.51 bifunctional enzyme, chorismate mutase:prephenate dehydratase Escherichia coli
5.4.99.5 chorismate mutase/prephenate dehydratase Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.51 169
-
-
Escherichia coli
5.4.99.5 additional information
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.2.1.51 4°C, 20 mM Tris-HCl, pH 8.2, 1 mM EDTA, 10 mM 2-mercaptoethanol, 0.3 M NaCl, 0.02% w/v NaN3, 1 mM dihiothreitol, 2 weeks stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.51 prephenate
-
Escherichia coli phenylpyruvate + H2O + CO2
-
?
4.2.1.51 prephenate
-
Escherichia coli ?
-
?
5.4.99.5 Chorismate
-
Escherichia coli Prephenate
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.51 dimer 2 * 43111, smallest native species of enzyme, calculation from nucleotide sequence Escherichia coli
4.2.1.51 octamer 8 * 43111, calculation from nucleotide sequence Escherichia coli
5.4.99.5 ? x * 40000, the smallest native species of the enzyme, determined by sedimentation equilibrium, appears to be a dimer Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.51 6.2 8.6
-
Escherichia coli