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Literature summary extracted from
- Properties of a 2,3-oxidosqualene-cycloartenol cyclase from Ochromonas malhamensis (1971), FEBS Lett., 18, 175-178.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.8 | 2,3-iminosqualene | - |
Ochromonas malhamensis |  |
| 5.4.99.8 | dithiothreitol | stimulates | Ochromonas malhamensis | |
| 5.4.99.8 | sodium deoxycholate | inhibition at levels greater than 0.5%, stimulates at low concentrations, optimal activation at 0.1% | Ochromonas malhamensis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.8 | KCl | stimulation at concentrations up to 0.35 M, at higher levels slight fall of activity | Ochromonas malhamensis | |
| 5.4.99.8 | p-hydroxymercuribenzoate | - |
Ochromonas malhamensis | |
| 5.4.99.8 | sodium deoxycholate | stimulates at low concentrations, optimal activation at 0.1%. Inhibition at levels greater than 0.5% | Ochromonas malhamensis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.8 | 0.1 | - |
(S)-2,3-epoxysqualene | - |
Ochromonas malhamensis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.8 | KCl | enzyme is inactive in absence of KCl, stimulation at concentrations up to 0.35 M, at higher levels slight fall of activity | Ochromonas malhamensis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.8 | Ochromonas malhamensis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.8 | - |
Ochromonas malhamensis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.8 | 0.00019 | - |
- |
Ochromonas malhamensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (S)-2,3-Epoxysqualene | - |
Ochromonas malhamensis | Cycloartenol | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.8 | 37 | - |
30 min incubation | Ochromonas malhamensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.8 | 7 | 7.3 | - |
Ochromonas malhamensis |
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Release 2023.1 (January 2023)
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