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Literature summary extracted from

  • Schmidt, E.; Knackmuss, H.J.
    Chemical structure and biodegradability of halogenated aromatic compounds. Conversion of chlorinated muconic acids into maleoylacetic acid (1980), Biochem. J., 192, 339-347.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.45 0.015
-
 trans-4-Carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii
3.1.1.45 0.4
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii
5.5.1.1 0.018
-
 3-methyl-cis,cis-muconate
-
 Pseudomonas sp.
5.5.1.1 0.0558
-
 cis,cis-muconate
-
 Pseudomonas sp.
5.5.1.1 0.105
-
 2-methyl-cis,cis-muconate
-
 Pseudomonas sp.
5.5.1.1 0.134
-
 3-chloro-cis,cis-muconate
-
 Pseudomonas sp.
5.5.1.1 0.415
-
 2-chloro-cis,cis-muconate
-
 Pseudomonas sp.
5.5.1.7 0.0134
-
 3-methyl-cis,cis-muconate
-
 Pseudomonas sp.
5.5.1.7 0.0309
-
 2-methyl-cis,cis-muconate
-
 Pseudomonas sp.
5.5.1.7 0.0601
-
 2-chloro-cis,cis-muconate
-
 Pseudomonas sp.
5.5.1.7 0.0928
-
 cis,cis-muconate
-
 Pseudomonas sp.
5.5.1.7 0.1286
-
 3-chloro-cis,cis-muconate
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.1 cis,cis-Muconate Pseudomonas sp. enzyme of the benzoate catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Pseudomonas knackmussii
-
-
-
5.5.1.1 Pseudomonas sp.
-
 B13
-
5.5.1.1 Pseudomonas sp. B13
-
 B13
-
5.5.1.7 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.45 3-chlorobenzoate-grown cells Pseudomonas knackmussii
5.5.1.7
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.45 205.5
-
-
 Pseudomonas knackmussii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
5.5.1.1 2-Chloro-cis,cis-muconate 0.8% of the activity relative to cis,cis-muconate Pseudomonas sp. ?
-
 ?
5.5.1.1 2-Chloro-cis,cis-muconate 0.8% of the activity relative to cis,cis-muconate Pseudomonas sp. B13 ?
-
 ?
5.5.1.1 2-Methyl-cis,cis-muconate 90% of the activity relative to cis,cis-muconate Pseudomonas sp. ?
-
 ?
5.5.1.1 2-Methyl-cis,cis-muconate 90% of the activity relative to cis,cis-muconate Pseudomonas sp. B13 ?
-
 ?
5.5.1.1 3-Chloro-cis,cis-muconate 7.1% of the activity relative to cis,cis-muconate Pseudomonas sp. 5-Chloromuconolactone
-
 ?
5.5.1.1 3-Chloro-cis,cis-muconate 7.1% of the activity relative to cis,cis-muconate Pseudomonas sp. B13 5-Chloromuconolactone
-
 ?
5.5.1.1 3-Methyl-cis,cis-muconate 11.7% of the activity relative to cis,cis-muconate Pseudomonas sp. beta-Methylmuconolactone
-
 ?
5.5.1.1 3-Methyl-cis,cis-muconate 11.7% of the activity relative to cis,cis-muconate Pseudomonas sp. B13 beta-Methylmuconolactone
-
 ?
5.5.1.1 cis,cis-Muconate
-
 Pseudomonas sp. (+)-Muconolactone
-
 ?
5.5.1.1 cis,cis-Muconate
-
 Pseudomonas sp. B13 (+)-Muconolactone
-
 ?
5.5.1.1 cis,cis-Muconate enzyme of the benzoate catabolism Pseudomonas sp. ?
-
 ?
5.5.1.7 2-Chloro-cis,cis-muconate 65% of the activity relative to 2-methyl-cis,cis-muconate Pseudomonas sp. 4-Carboxymethyl-2-chloro-but-2-en-4-olide trans-4-carboxymethylenebut-2-en-4-olide is produced after dehalogenation ?
5.5.1.7 2-Methyl-cis,cis-muconate most effective substrate Pseudomonas sp. ?
-
 ?
5.5.1.7 3-Chloro-cis,cis-muconate 73% of the activity relative to 2-methyl-cis,cis-muconate Pseudomonas sp. cis-4-Carboxymethylenebut-2-en-4-olide
-
 ?
5.5.1.7 3-Methyl-cis,cis-muconate 84% of the activity relative to 2-methyl-cis,cis-muconate Pseudomonas sp. ?
-
 ?
5.5.1.7 cis,cis-Muconate 36% of the activity relative to 2-methyl-cis,cis-muconate Pseudomonas sp. ?
-
 ?