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Literature summary extracted from
- Characterization of the 30-kDa enzyme from red blood cells that cleaves ubiquitin-protein conjugates (1994), Biochem. Biophys. Res. Commun., 205, 354-360.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.12 | iodoacetamide | - |
Bos taurus |  |
| 3.4.19.12 | iodoacetamide | - |
Homo sapiens | |
| 3.4.19.12 | Ubiquitin aldehyde | - |
Bos taurus | |
| 3.4.19.12 | ubiquitin-aldehyde | - |
Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 30000 | - |
gel filtration | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.12 | Bos taurus | - |
- |
- |
| 3.4.19.12 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.12 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.19.12 | erythrocyte | - |
Homo sapiens | - |
| 3.4.19.12 | erythrocyte | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 14.75 | - |
purified enzyme, substrate A24 | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.12 | conjugate of histone H2A and ubiquitin + H2O | best substrate | Bos taurus | ubiquitin + histone H2A | - |
? | |
| 3.4.19.12 | conjugate of histone H2A and ubiquitin + H2O | i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds | Bos taurus | ubiquitin + histone H2A | - |
? | |
| 3.4.19.12 | additional information | enzyme contains ubiquitin-C-terminal hydrolase activity towards biosynthetic precursors of ubiquitin as well as isopeptidase activity towards ubiquitin histone conjugates as human ubiquitin carboxyl extension protein and ubiquitin C-terminal amide | Bos taurus | ? | - |
? | |
| 3.4.19.12 | ubiquitin C-terminal amide + H2O | - |
Homo sapiens | ubiquitin + NH3 | - |
? | |
| 3.4.19.12 | ubiquitin fused to 60% of its N-terminus + H2O | recombinant substrate, expressed in Escherichia coli | Bos taurus | ubiquitin + 60% of ubiquitin N-terminus | - |
? | |
| 3.4.19.12 | ubiquitin metallothionein + H2O | low activity | Bos taurus | ? | - |
? | |
| 3.4.19.12 | ubiquitin metallothionein + H2O | recombinant substrate, expressed in Escherichia coli | Bos taurus | ? | - |
? | |
| 3.4.19.12 | ubiquitin metallothionein + H2O | i.e. UbMT | Bos taurus | ? | - |
? | |
| 3.4.19.12 | ubiquitin-carboxyl extension protein + H2O | carboxyl extension protein of 52 amino acids | Homo sapiens | ubiquitin + ubiquitin-carboxyl extension protein | - |
? | |
| 3.4.19.12 | ubiquitin-human carboxyl extension protein fusion protein + H2O | i.e. HUbCEP-52 | Bos taurus | ubiquitin + human carboxyl extension protein | - |
? | |
| 3.4.19.12 | ubiquitin-human carboxyl extension protein fusion protein + H2O | recombinant substrate, expressed in Escherichia coli | Bos taurus | ubiquitin + human carboxyl extension protein | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 37 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 37 | - |
60 min, 70% loss of activity towards A24, no loss of activity towards ubiquitin C-terminal amide and human ubiquitin carboxyl extension protein | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 7.2 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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