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Literature summary extracted from
- Purification and characterization or porcine kidney cathepsin B (1981), J. Biochem., 90, 1677-1684.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.1 | 1-tosylamide-2-phenylethyl chloromethylketone | - |
Sus scrofa |  |
| 3.4.22.1 | antipain | - |
Sus scrofa | |
| 3.4.22.1 | iodoacetamide | - |
Sus scrofa | |
| 3.4.22.1 | iodoacetic acid | - |
Sus scrofa | |
| 3.4.22.1 | leupeptin | Ki: 0.15-0.17 nM | Sus scrofa | |
| 3.4.22.1 | N-ethylmaleimide | - |
Sus scrofa | |
| 3.4.22.1 | N-tosyl-lysyl chloromethylketone | - |
Sus scrofa | |
| 3.4.22.1 | phenylmethanesulfonyl fluoride | no inhibition at 0.5 mM | Sus scrofa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.1 | 0.204 | 0.225 | benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | - |
Sus scrofa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 24000 | 31000 | isoenzyme I, gel filtration, SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | Proteins + H2O | Sus scrofa | turnover of intracellular proteins | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.1 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.1 | - |
Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.1 | kidney | - |
Sus scrofa | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.22.1 | -20°C, 4 months, stable | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide | - |
Sus scrofa | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.1 | Proteins + H2O | turnover of intracellular proteins | Sus scrofa | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.1 | 63 | 71 | benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | - |
Sus scrofa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 6 | - |
- |
Sus scrofa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 4 | 6.7 | stable | Sus scrofa |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.1 | 0.000015 | 0.000017 | leupeptin | - |
Sus scrofa | |
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