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Literature summary extracted from

  • Barrett, A.J.; Brown, M.A.; Dando, P.M.; Knight, C.G.; McKie, N.; Rawlings, N.D.; Serizawa, A.
    Thimet oligopeptidase and oligopeptidase M or neurolysin (1995), Methods Enzymol., 248, 529-556.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.15 2-mercaptoethanol enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of 2-mercaptoethanol Homo sapiens
3.4.24.15 2-mercaptoethanol enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of 2-mercaptoethanol Rattus norvegicus
3.4.24.15 dithiothreitol enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of dithiothreitol Homo sapiens
3.4.24.15 dithiothreitol enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of dithiothreitol Rattus norvegicus
3.4.24.16 thiols activate Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.15
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.15 dithiothreitol 10 mM, reversible Homo sapiens
3.4.24.15 dithiothreitol 10 mM, reversible Rattus norvegicus
3.4.24.15 EDTA
-
 Homo sapiens
3.4.24.15 EDTA reactivation by metal ions Rattus norvegicus
3.4.24.15 Hg2+
-
 Homo sapiens
3.4.24.15 Hg2+
-
 Rattus norvegicus
3.4.24.15 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-p-aminobenzoate
-
 Homo sapiens
3.4.24.15 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-p-aminobenzoate
-
 Rattus norvegicus
3.4.24.15 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate
-
 Rattus norvegicus
3.4.24.15 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-p-aminobenzoate
-
 Homo sapiens
3.4.24.15 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-p-aminobenzoate
-
 Rattus norvegicus
3.4.24.15 p-hydroxymercuribenzoate
-
 Homo sapiens
3.4.24.15 p-hydroxymercuribenzoate
-
 Rattus norvegicus
3.4.24.15 Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys i.e. dynorphin-(1-13) Homo sapiens
3.4.24.15 Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys i.e. dynorphin-(1-13) Rattus norvegicus
3.4.24.15 Zn2+ above 0.1 mM Homo sapiens
3.4.24.15 Zn2+ above 0.1 mM Rattus norvegicus
3.4.24.16 1,10-phenanthroline
-
 Rattus norvegicus
3.4.24.16 dynorphin A(1-13)
-
 Rattus norvegicus
3.4.24.16 EDTA
-
 Rattus norvegicus
3.4.24.16 N-(phenylethylphosphonyl)-Gly-Pro-L-norleucine
-
 Rattus norvegicus
3.4.24.16 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-4-aminobenzoate
-
 Rattus norvegicus
3.4.24.16 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-4-aminobenzoate
-
 Rattus norvegicus
3.4.24.16 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-4-aminobenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.15 0.0014
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl recombinant enzyme from E. coli Rattus norvegicus
3.4.24.15 0.0025
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl recombinant enzyme from E. coli Rattus norvegicus
3.4.24.15 0.009
-
 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
-
 Homo sapiens
3.4.24.15 0.012
-
 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Rattus norvegicus
3.4.24.15 0.0133
-
 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Homo sapiens
3.4.24.15 0.0161
-
 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys recombinant enzyme from E. coli Rattus norvegicus
3.4.24.15 0.023
-
 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
-
 Rattus norvegicus
3.4.24.15 0.0343
-
 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl recombinant enzyme from E. coli Rattus norvegicus
3.4.24.16 0.0022
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl
-
 Rattus norvegicus
3.4.24.16 0.0043
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl
-
 Rattus norvegicus
3.4.24.16 0.0498
-
 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl abvove Rattus norvegicus
3.4.24.16 0.05
-
 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.16 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.15 Zinc contains 2 gatom of zinc per mol of enzyme, one catalytic zinc and one structural zinc Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.15 78300
-
 x * 78300, calculation from nucleotide sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.15 additional information Homo sapiens proposed role in formation of enkephalins ?
-
 ?
3.4.24.15 additional information Rattus norvegicus proposed role in formation of enkephalins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.15 Homo sapiens
-
-
-
3.4.24.15 Rattus norvegicus
-
-
-
3.4.24.16 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.15
-
 Homo sapiens
3.4.24.16
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.15 erythrocyte
-
 Homo sapiens
-
3.4.24.15 hypophysis
-
 Rattus norvegicus
-
3.4.24.15 testis
-
 Rattus norvegicus
-
3.4.24.16 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.15 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.15 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl
-
 Rattus norvegicus (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile + Arg-Arg-Ala-Lys-dinitrophenyl
-
 ?
3.4.24.15 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl
-
 Rattus norvegicus (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu + Arg-Arg-Ala-Lys-dinitrophenyl
-
 ?
3.4.24.15 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg
-
 Homo sapiens 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg
-
 ?
3.4.24.15 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg
-
 Rattus norvegicus 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg
-
 ?
3.4.24.15 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
-
 Homo sapiens 7-methoxycoumarin-3-carboxylyl-Pro-Leu + Gly-Pro-D-Lys-dinitrophenyl
-
 ?
3.4.24.15 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
-
 Rattus norvegicus 7-methoxycoumarin-3-carboxylyl-Pro-Leu + Gly-Pro-D-Lys-dinitrophenyl
-
 ?
3.4.24.15 benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
 Homo sapiens benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate
-
 ?
3.4.24.15 benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
 Rattus norvegicus benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate
-
 ?
3.4.24.15 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Homo sapiens dinitrophenyl-Pro-Leu + Gly-Pro-Trp-D-Lys
-
 ?
3.4.24.15 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Rattus norvegicus dinitrophenyl-Pro-Leu + Gly-Pro-Trp-D-Lys
-
 ?
3.4.24.15 additional information proposed role in formation of enkephalins Homo sapiens ?
-
 ?
3.4.24.15 additional information proposed role in formation of enkephalins Rattus norvegicus ?
-
 ?
3.4.24.15 pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu i.e. neurotensin Homo sapiens pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu
-
 ?
3.4.24.15 pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu i.e. neurotensin Rattus norvegicus pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu
-
 ?
3.4.24.15 Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O dynorphin 1-17 Homo sapiens ?
-
 ?
3.4.24.15 Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O dynorphin 1-17 Rattus norvegicus ?
-
 ?
3.4.24.16 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl + H2O i.e. QF37, cleavage site: Arg6-Ala7 Rattus norvegicus (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg + Ala-Lys-dinitrophenyl
-
 ?
3.4.24.16 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl + H2O i.e. QF34, cleavage sites: Leu4-Arg5, Arg5-Arg6 Rattus norvegicus peptide fragments
-
 ?
3.4.24.16 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.16 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys + H2O
-
 Rattus norvegicus peptide fragments
-
 ?
3.4.24.16 pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
-
 Rattus norvegicus pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
-
 ?
3.4.24.16 Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O i.e. dynorphin A(1-17), cleavage sites: Lys11-Leu12, Leu12-Lys13 Rattus norvegicus peptide fragments
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.15 monomer x * 78300, calculation from nucleotide sequence Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.16 thimet peptidase II
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.15 8.2
-
 above Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.15 8
-
 unstable above Rattus norvegicus