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Literature summary extracted from

  • Lessley, B.A.; Garner, D.L.
    Purification and characterization of Pz-peptidase B, a neutral metalloendopeptidase from bovine spermatozoa (1990), Biol. Reprod., 43, 643-658.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.15 EDTA reactivation by metal ions Bos taurus
3.4.24.15 phosphoramidon
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.15 90700
-
 2 * 90700, SDS-PAGE Bos taurus
3.4.24.15 184000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.15 Bos taurus
-
 Pz peptidase A and B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.15
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.15 spermatozoon
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
3.4.24.15 5°C or -70°C, 3 months, more than 95% of the activity is retained Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.15 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg
-
 Bos taurus 4-phenylazobenzyloxycarbonyl-Pro + Leu-Gly-Pro-D-Arg
-
 ?
3.4.24.15 aminobenzyl-Ala-Gly-Leu-Ala-nitrobenzylamide weak Bos taurus aminobenzyl-Ala-Gly + Leu-Ala-nitrobenzylamide
-
 ?
3.4.24.15 Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
-
 Bos taurus Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
 ?
3.4.24.15 succinyl-Ala-Ala-Ala-4-nitroanilide weak Bos taurus succinyl-Ala-Ala + Ala-4-nitroanilide
-
 ?
3.4.24.15 succinyl-Ala-Ala-Phe-4-nitroanilide weak Bos taurus succinyl-Ala-Ala + Phe-4-nitroanilide
-
 ?
3.4.24.15 succinyl-Ala-Ala-Val-4-nitroanilide weak Bos taurus succinyl-Ala-Ala + Val-4-nitroanilide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.15 dimer 2 * 90700, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.15 7.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.15 6.5 8.5 more than 50% of maximal activity at pH 6.5 and at pH 8.5 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.15 3.5
-
 irreversible inactivation at or below Bos taurus