Literature summary extracted from

Terai, Y.; Fujii, I.; Byun, S.H.; Nakajima, O.; Hakamatsuka, T.; Ebizuka, Y.; Sankawa, U.
Cloning of chalcone-flavanone isomerase cDNA from Pueraria lobata and its overexpression in Escherichia coli (1996), Protein Expr. Purif., 8, 183-190.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
5.5.1.6	overexpression in Escherichia coli	Pueraria montana var. lobata

Protein Variants

EC Number	Protein Variants	Comment	Organism
5.5.1.6	C119A	mutant enzyme Cys119Ala, is less sensitive to SH-inhibitors, shows chalcone isomerase activity	Pueraria montana var. lobata

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
5.5.1.6	HgCl2	mutant enzyme Cys119Ala, is less sensitive than wild type enzyme	Pueraria montana var. lobata
5.5.1.6	iodoacetamide	mutant enzyme Cys119Ala, is less sensitive than wild type enzyme	Pueraria montana var. lobata
5.5.1.6	NEM	mutant enzyme Cys119Ala, is less sensitive than wild type enzyme	Pueraria montana var. lobata

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.5.1.6	Pueraria montana var. lobata	Q43056	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
5.5.1.6	recombinant enzyme	Pueraria montana var. lobata

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.5.1.6	additional information	-	-	Pueraria montana var. lobata

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Release 2023.1 (January 2023)