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Literature summary extracted from

  • Tardy, F.; Nasser, W.; Robert-Baudouy, J.; Hugouvieux-Cotte-Pattat, N.
    Comparative analysis of the five major Erwinia chrysanthemi pectate lyases: enzyme characteristics and potential inhibitors (1997), J. Bacteriol., 179, 2503-2511.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2
-
 Dickeya chrysanthemi

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Ba2+
-
 Dickeya chrysanthemi
4.2.2.2 Ca2+ 1 mM, strong inhibition of isoenzyme pelE Dickeya chrysanthemi
4.2.2.2 Co2+
-
 Dickeya chrysanthemi
4.2.2.2 epicatechin
-
 Dickeya chrysanthemi
4.2.2.2 Mn2+ 1 mM, weak Dickeya chrysanthemi
4.2.2.2 salicylic acid
-
 Dickeya chrysanthemi
4.2.2.2 Sr2+ 1 mM, weak Dickeya chrysanthemi
4.2.2.2 Zn2+
-
 Dickeya chrysanthemi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information
-
 Dickeya chrysanthemi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ activates Dickeya chrysanthemi
4.2.2.2 Ca2+ optimal concentration: 0.1 mM Dickeya chrysanthemi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 39500
-
 x * 39500, isoenzymes PelB and PelC, SDS-PAGE Dickeya chrysanthemi
4.2.2.2 42000
-
 x * 42000, isoenzyme PelD, SDS-PAGE Dickeya chrysanthemi
4.2.2.2 42500
-
 x * 42500, isoenzymes PelA and PelE, SDS-PAGE Dickeya chrysanthemi

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Dickeya chrysanthemi
-
 5 isoenzymes: PelA, PelB, PelC, PelD, PelE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 5 isoenzymes: PelA, PelB, PelC, PelD, PelE Dickeya chrysanthemi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.2 additional information
-
-
 Dickeya chrysanthemi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 polygalacturonic acid
-
 Dickeya chrysanthemi unsaturated oligogalacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 42500, isoenzymes PelA and PelE, SDS-PAGE Dickeya chrysanthemi
4.2.2.2 ? x * 42000, isoenzyme PelD, SDS-PAGE Dickeya chrysanthemi
4.2.2.2 ? x * 39500, isoenzymes PelB and PelC, SDS-PAGE Dickeya chrysanthemi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 50
-
 isoenzyme PelD and pelE Dickeya chrysanthemi
4.2.2.2 55
-
 isoenzyme, isoenzyme A Dickeya chrysanthemi
4.2.2.2 60
-
 isoenzyme PelB pelC Dickeya chrysanthemi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 50
-
 half life: 11 min for isoenzyme PelA, 4 min for isoenzyme PelD, 2 min for isoenzyme PelE Dickeya chrysanthemi
4.2.2.2 60
-
 half-life: 20-30 min for isoenzyme PelB and PelC, isoenzymes PelA, PelD and PelE loses more than 80% of activity after 2 min Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8
-
 isoenzyme pelE Dickeya chrysanthemi
4.2.2.2 8.5
-
 isoenzyme PelA Dickeya chrysanthemi
4.2.2.2 8.8
-
 isoenzyme isoenzyme pelD Dickeya chrysanthemi
4.2.2.2 9.2
-
 isoenzyme PelC Dickeya chrysanthemi
4.2.2.2 9.3
-
 isoenzyme PelB Dickeya chrysanthemi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 additional information
-
-
 Dickeya chrysanthemi