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Literature summary extracted from
- Metabolism of 2-ketoaldehydes in mold: purification and characterization of glyoxalase I from Aspergillus niger (1987), J. Biochem., 102, 583-589.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.5 | dithiothreitol | slight | Aspergillus niger |  |
| 4.4.1.5 | EDTA | - |
Aspergillus niger | |
| 4.4.1.5 | iodoacetamide | slight | Aspergillus niger | |
| 4.4.1.5 | Mg2+ | - |
Aspergillus niger | |
| 4.4.1.5 | Zn2+ | 0.5 mM, complete inhibition | Aspergillus niger | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.5 | 0.87 | - |
4,5-Dioxovalerate | - |
Aspergillus niger | |
| 4.4.1.5 | 1.25 | - |
methylglyoxal | - |
Aspergillus niger | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.5 | Fe2+ | 0.5 mM activates 2.4fold | Aspergillus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 36000 | - |
gel filtration | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.5 | Aspergillus niger | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.5 | - |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 22.7 | - |
- |
Aspergillus niger |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.4.1.5 | 4°C, stable for 3 weeks | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.5 | glutathione + 4,5-dioxovalerate | - |
Aspergillus niger | ? | - |
? | |
| 4.4.1.5 | glutathione + methylglyoxal | - |
Aspergillus niger | S-lactoylglutathione | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.5 | monomer | 1 * 36000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 35 | 37 | - |
Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 7 | - |
- |
Aspergillus niger |
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Release 2023.1 (January 2023)
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