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Literature summary extracted from

  • Caballero, F.J.; Cejudo, F.J.; Florencio, F.J.; Cardenas, J.; Castillo, F.
    Molecular and regulatory properties of glutamine synthetase from the phototrophic bacterium Rhodopseudomonas capsulata E1F1 (1985), J. Bacteriol., 162, 804-809.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 Ala strong inhibition of transferase activity Rhodobacter capsulatus
6.3.1.2 Asp inhibition of biosynthetic and transferase activity Rhodobacter capsulatus
6.3.1.2 Gly inhibition of biosynthetic and transferase activity Rhodobacter capsulatus
6.3.1.2 Ser inhibition of biosynthetic and transferase activity Rhodobacter capsulatus
6.3.1.2 Val inhibition of biosynthetic and transferase activity Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.01
-
 ADP transferase activity Rhodobacter capsulatus
6.3.1.2 6
-
 hydroxylamine transferase activity Rhodobacter capsulatus
6.3.1.2 12
-
 L-Gln transferase activity Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 55000
-
 12 * 55000, the enzyme exhibits different aggregation states with detectable oligomers of 1, 2, 3, 4, 6, 8, 10, and 12 subunits, SDS-PAGE Rhodobacter capsulatus
6.3.1.2 660000 670000 disc gel electrophoresis, pore gradient electrophoresis, sucrose density gradient centrifugation Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Rhodobacter capsulatus
-
-
-
6.3.1.2 Rhodobacter capsulatus E1F1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
 Rhodobacter capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 153
-
 gamma-glutamyl transferase assay Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Rhodobacter capsulatus ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-Glu + NH4+
-
 Rhodobacter capsulatus E1F1 ADP + phosphate + L-Gln
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Rhodobacter capsulatus gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Rhodobacter capsulatus E1F1 gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 dodecamer 12 * 55000, the enzyme exhibits different aggregation states with detectable oligomers of 1, 2, 3, 4, 6, 8, 10, and 12 subunits, SDS-PAGE Rhodobacter capsulatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.2 additional information
-
 additional information turnover number 4200, biosynthetic activity. 102000, transferase activity Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 6.5
-
 adenylylated form, n:9, transferase activity Rhodobacter capsulatus
6.3.1.2 7
-
 intermediate form, n:6, transferase activity Rhodobacter capsulatus
6.3.1.2 7.5
-
 deadenylylated form, transferase activity Rhodobacter capsulatus