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Literature summary extracted from

  • Ertan, H.
    Some properties of glutamate dehydrogenase, glutamine synthetase and glutamate synthase from Corynebacterium callunae (1992), Arch. Microbiol., 158, 35-41.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 Ala
-
 Corynebacterium callunae
6.3.1.2 AMP no inhibition Corynebacterium callunae
6.3.1.2 ATP
-
 Corynebacterium callunae
6.3.1.2 Co2+ 0.5-60 mM, inhibition of Mn2+-dependent transferase activity Corynebacterium callunae
6.3.1.2 Gly
-
 Corynebacterium callunae
6.3.1.2 Mg2+ between 0.5-60 mM, inhibition of Mn2+-dependent transferase activity Corynebacterium callunae
6.3.1.2 Ni2+ 0.5-60 mM, inhibition of Mn2+-dependent transferase activity Corynebacterium callunae
6.3.1.2 Ser
-
 Corynebacterium callunae
6.3.1.2 Zn2+ 0.5-60 mM, inhibition of Mn2+-dependent transferase activity Corynebacterium callunae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.0035
-
 ADP
-
 Corynebacterium callunae
6.3.1.2 1.46
-
 hydroxylamine
-
 Corynebacterium callunae
6.3.1.2 15.2
-
 Gln
-
 Corynebacterium callunae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 K+ strongly stimulates transferase activity Corynebacterium callunae
6.3.1.2 Mg2+ divalent cation required, specificity for Mg2+ in the biosynthetic assay Corynebacterium callunae
6.3.1.2 Mg2+ maximal activity at 30 mM Corynebacterium callunae
6.3.1.2 Mn2+ maximal activity at 1 mM Corynebacterium callunae
6.3.1.2 Mn2+ required for transferase activity Corynebacterium callunae

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Corynebacterium callunae
-
-
-
6.3.1.2 Corynebacterium callunae NCIB 10338
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Corynebacterium callunae ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-Glu + NH4+
-
 Corynebacterium callunae NCIB 10338 ADP + phosphate + L-Gln
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP maximal transferase activity with ADP, and good activity with AMP and GDP Corynebacterium callunae gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP maximal transferase activity with ADP, and good activity with AMP and GDP Corynebacterium callunae NCIB 10338 gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 45
-
 biosynthetic activity Corynebacterium callunae
6.3.1.2 55
-
 transferase activity Corynebacterium callunae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.2 60
-
 20 min, 20% loss of activity Corynebacterium callunae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7
-
 biosynthetic assay Corynebacterium callunae
6.3.1.2 8
-
 transferase activity Corynebacterium callunae