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Literature summary extracted from

  • MacKintosh, R.W.; Fewson, C.A.
    Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II from Acinetobacter calcoaceticus. Substrate specificities and inhibition studies (1988), Biochem. J., 255, 653-661.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.90 2-Bromobenzaldehyde 0.01 mM, 88% inhibition Acinetobacter calcoaceticus
1.1.1.90 2-Chlorobenzaldehyde 0.01 mM, 95% inhibition Acinetobacter calcoaceticus
1.1.1.90 2-Fluorobenzaldehyde 0.1 mM, 100% inhibition Acinetobacter calcoaceticus
1.1.1.90 3,4-dimethoxybenzaldehyde 0.1 mM, 10% inhibition Acinetobacter calcoaceticus
1.1.1.90 3-methylbenzaldehyde 0.1 mM, 75% inhibition Acinetobacter calcoaceticus
1.1.1.90 4-Isopropylbenzaldehyde 0.01 mM, 87% inhibition Acinetobacter calcoaceticus
1.1.1.90 iodoacetamide slight Acinetobacter calcoaceticus
1.1.1.90 iodoacetate
-
 Acinetobacter calcoaceticus
1.1.1.90 N-ethylmaleimide
-
 Acinetobacter calcoaceticus
1.1.1.90 p-chloromercuribenzoate
-
 Acinetobacter calcoaceticus
1.1.1.90 pentafluorobenzaldehyde 0.1 mM, 44% inhibition Acinetobacter calcoaceticus
1.2.1.28 2-Bromobenzaldehyde 0.1 mM, 90% inhibition, benzaldehyde dehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 2-Chlorobenzaldehyde 0.1 mM, 100% inhibition, benzaldehydedehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 2-Fluorobenzaldehyde 0.1 mM, 100% inhibition, benzaldehydedehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 3-methylbenzaldehyde 0.1 mM, 75% inhibition, benzaldehyde dehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 4-chloromercuribenzoate 0.005 mM, 50% inhibition after 270 min, benzaldehyde dehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 4-Isopropylbenzaldehyde 0.01 mM, 87% inhibition, benzaldehyde dehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 iodoacetamide 1 mM, 50% inhibition after 210 min, benzaldehyde dehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 iodoacetate 10 mM, 50% inhibition after 30 min, benzaldehyde dehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 N-ethylmaleimide 1 mM, 50% inhibition after 160 min, benzaldehyde dehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 pentafluorobenzaldehyde 0.1 mM, 44% inhibition, benzaldehyde dehydrogenase II Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.018
-
 perillyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.026
-
 benzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.044
-
 coniferyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.046
-
 4-Hydroxybenzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.053
-
 3,4-dimethoxybenzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.057
-
 4-methoxybenzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.09
-
 2-hydroxybenzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.093
-
 cinnamyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.111
-
 2-Methoxybenzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.111
-
 4-Isopropylbenzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.111
-
 thiophen-2-methanol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.145
-
 3-Methoxybenzyl alcohol
-
 Acinetobacter calcoaceticus
1.2.1.28 0.00057
-
 benzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.0015
-
 cinnamaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.0028
-
 4-Fluorobenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.0039
-
 3-Fluorobenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.0051
-
 thiophen-2-carboxaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.0065
-
 3-Methoxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.016
-
 4-methoxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.018
-
 4-hydroxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.02
-
 pyridine-4-carboxaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.026
-
 Pyridine-3-carboxaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.059
-
 furan-2-carboxyaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.063
-
 3-Hydroxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.072
-
 hexan-1-al
-
 Acinetobacter calcoaceticus
1.2.1.28 0.152
-
 perillaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.187
-
 2-Hydroxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.46
-
 octan-1-al
-
 Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.90 benzyl alcohol + NAD+ Acinetobacter calcoaceticus
-
 benzaldehyde + NADH
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus
-
 benzoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Acinetobacter calcoaceticus
-
-
-
1.2.1.28 Acinetobacter calcoaceticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 2-hydroxybenzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus 2-hydroxybenzaldehyde + NADH
-
 r
1.1.1.90 2-hydroxymethylthiophene + NAD+
-
 Acinetobacter calcoaceticus 2-formylthiophene + NADH
-
 r
1.1.1.90 2-methoxybenzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus 2-methoxybenzaldehyde + NADH
-
 r
1.1.1.90 3,4-dimethoxybenzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus 3,4-dimethoxybenzaldehyde + NADH
-
 r
1.1.1.90 3-methoxybenzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus 3-methoxybenzaldehyde + NADH
-
 ?
1.1.1.90 4-hydroxy-3-methoxybenzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus 4-hydroxy-3-methoxybenzaldehyde + NADH
-
 r
1.1.1.90 4-hydroxybenzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus 4-hydroxybenzaldehyde + NADH
-
 r
1.1.1.90 4-isopropylbenzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus 4-isopropylbenzaldehyde + NADH
-
 r
1.1.1.90 4-methoxybenzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus 4-methoxybenzaldehyde + NADH
-
 r
1.1.1.90 benzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus benzaldehyde + NADH
-
 ?
1.1.1.90 cinnamyl alcohol + NAD+
-
 Acinetobacter calcoaceticus cinnamic aldehyde + NADH
-
 r
1.1.1.90 coniferyl alcohol + NAD+
-
 Acinetobacter calcoaceticus coniferyl aldehyde + NADH + H+
-
 r
1.1.1.90 furan-2-methanol + NAD+
-
 Acinetobacter calcoaceticus 2-formylfuran + NADH
-
 r
1.1.1.90 additional information alcohols that are not oxidized Acinetobacter calcoaceticus ?
-
 ?
1.1.1.90 perillyl alcohol + NAD+
-
 Acinetobacter calcoaceticus perillaldehyde + NADH
-
 r
1.2.1.28 2-hydroxybenzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus 2-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.28 3-fluorobenzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus 3-fluorobenzoate + NADH + H+
-
 ?
1.2.1.28 3-hydroxybenzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus 3-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.28 3-methoxybenzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus 3-methoxybenzoate + NADH
-
 ?
1.2.1.28 4-fluorobenzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus 4-fluorobenzoate + NADH + H+
-
 ?
1.2.1.28 4-hydroxybenzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus 4-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.28 4-methoxybenzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus 4-methoxybenzoate + NADH + H+
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II exhibits esterase activity with 4-nitrophenyl acetate Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ?
1.2.1.28 cinnamaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus cinnamate + NADH + H+
-
 ?
1.2.1.28 furan-2-carboxyaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus furan-2-formiate + NADH
-
 ?
1.2.1.28 hexan-1-al + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus hexanoate + NADH
-
 ?
1.2.1.28 octan-1-al + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus octanoate + NADH
-
 ?
1.2.1.28 perillaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus perillic acid + NADH
-
 ?
1.2.1.28 pyridin-3-carboxyaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus pyridin-3-formiate + NADH
-
 ?
1.2.1.28 pyridine 4-carboxyaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus pyridine 4-formiate + NADH
-
 ?
1.2.1.28 thiophen-2-carboxaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus thiophen-2-formiate + NADH
-
 r
1.2.1.28 thiophen-2-carboxyaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II Acinetobacter calcoaceticus thiophen-2-formiate + NADH
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.90 80
-
 4-Hydroxybenzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 83
-
 perillyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 116
-
 4-methoxybenzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 131
-
 benzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 141
-
 cinnamyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 144
-
 4-Isopropylbenzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 156
-
 3-Methoxybenzyl alcohol
-
 Acinetobacter calcoaceticus
1.2.1.28 17
-
 perillaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 20
-
 4-hydroxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 26
-
 cinnamaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 26
-
 octan-1-al
-
 Acinetobacter calcoaceticus
1.2.1.28 29
-
 3-Hydroxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 29
-
 hexan-1-al
-
 Acinetobacter calcoaceticus
1.2.1.28 33
-
 3-Methoxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 41
-
 2-Hydroxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 41
-
 3-Fluorobenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 46
-
 furan-2-carboxaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 49
-
 4-methoxybenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 52
-
 thiophen-2-carboxyaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 64
-
 4-Fluorobenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 66
-
 pyridine-4-carboxaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 68
-
 Pyridine-3-carboxaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 72
-
 benzaldehyde
-
 Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 NAD+
-
 Acinetobacter calcoaceticus
1.1.1.90 NADH
-
 Acinetobacter calcoaceticus
1.2.1.28 NAD+
-
 Acinetobacter calcoaceticus