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Literature summary extracted from

  • Oikawa, T.; Nakai, J.; Tsukagawa, Y.; Soda, K.
    A novel type of D-mannitol dehydrogenase from Acetobacter xylinum: occurrence, purification, and basic properties (1997), Biosci. Biotechnol. Biochem., 61, 1778-1782.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.2 Hg2+ strong inhibitor Komagataeibacter xylinus
1.1.2.2 p-quinone
-
 Komagataeibacter xylinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.2 0.098
-
 D-mannitol with phenanzine methosulfate as electron acceptor Komagataeibacter xylinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.2 20000
-
 alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE Komagataeibacter xylinus
1.1.2.2 51000
-
 alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE Komagataeibacter xylinus
1.1.2.2 69000
-
 alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE Komagataeibacter xylinus
1.1.2.2 140000
-
 gel filtration Komagataeibacter xylinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.2 D-mannitol + ferricytochrome c Komagataeibacter xylinus
-
 D-fructose + ferrocytochrome c
-
 ?
1.1.2.2 D-mannitol + ferricytochrome c Komagataeibacter xylinus KU-1
-
 D-fructose + ferrocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.2 Komagataeibacter xylinus
-
-
-
1.1.2.2 Komagataeibacter xylinus KU-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.2
-
 Komagataeibacter xylinus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.2 213
-
-
 Komagataeibacter xylinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.2 acyclic D-erythro polyols + ferricytochrome c no other substances than D-mannitol and D-sorbitol are accepted as substrates Komagataeibacter xylinus acyclic D-erythro ketoses + ferrocytochrome c
-
 ?
1.1.2.2 acyclic D-erythro polyols + ferricytochrome c no other substances than D-mannitol and D-sorbitol are accepted as substrates Komagataeibacter xylinus KU-1 acyclic D-erythro ketoses + ferrocytochrome c
-
 ?
1.1.2.2 D-mannitol + ferricytochrome c
-
 Komagataeibacter xylinus D-fructose + ferrocytochrome c
-
 ?
1.1.2.2 D-mannitol + ferricytochrome c
-
 Komagataeibacter xylinus KU-1 D-fructose + ferrocytochrome c
-
 ?
1.1.2.2 D-sorbitol + ferricytochrome c
-
 Komagataeibacter xylinus D-fructose + ferrocytochrome c
-
 ?
1.1.2.2 D-sorbitol + ferricytochrome c
-
 Komagataeibacter xylinus KU-1 D-fructose + ferrocytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.2 trimer alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE Komagataeibacter xylinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.2 40
-
-
 Komagataeibacter xylinus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.2.2 20 60
-
 Komagataeibacter xylinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.2.2 50
-
 stable up to 50°C at pH 6 Komagataeibacter xylinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.2 5
-
-
 Komagataeibacter xylinus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.2.2 3 8
-
 Komagataeibacter xylinus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.2.2 4 7 stable for 30 min at 30°C Komagataeibacter xylinus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.2 cytochrome c
-
 Komagataeibacter xylinus
1.1.2.2 additional information no activity with NAD+ and NADP+ Komagataeibacter xylinus
1.1.2.2 phenazine methosulfate suitable as electron acceptor Komagataeibacter xylinus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.2.2 0.18
-
 p-quinone
-
 Komagataeibacter xylinus